The Incidence and Evaluation of Diagnostic Methods used for Detection of Helicobacter Pylori in Wad Madani Teaching Hospital, Gezira State, Sudan (2020)

الموضوعات

طب

الملخص

The gastrointestinal tract infection is considered as one of the most common diseases that affect human health with serious consequences and many complications. Helicobacter pylori infect nearly 50% of the world’s population. This microorganism is considered as the most important cause of gastritis, as a risk factor for peptic ulcer disease and consequently gastric adenocarcinoma. The objective of the current study was to evaluate the incidence of H. pylori among patients who attended to the Gastroendoscopy Department, wad Madani Teaching Hospital to be investigated for suspected infection, as well as to evaluate the diagnostic methods used for the detection of H. pylori in the gastric biopsy specimens collected from these patients .These methods were rapid urease test, histopathology , and polymerase chain reaction (PCR). This research work also aimed at testing the molecular characterization of 16S rRNA gene. The research project was a prospective cross -sectional study carried out in the Gezira State, between December 2015 to October 2019. One hundred participants from both genders were recruited in the study according to the inclusion and exclusion criteria. The results showed that the incidence of H. pylori was higher in males compared to females. Furthermore, they showed that nausea, loss of weight, and bad breath odor, were significantly associated with H. pylori infection. According to the results the conventional PCR it exhibits 79% sensitivity and 100% specificity, RUT showed 72% sensitivity and 100% specificity and histopathology showed 100% sensitivity 95% specificity. Four of the sample sequences which were subjected to BLAST analysis showed close similarity to the GenBank strains of H. pylori. The alignment of these sample sequences showed that Sudanese H. pylori strain in this study exhibited difference on one base-pair insertion of cytosine(C). It was concluded that; this study showed a high level of H. pylori among the study population which is a proper indication for a high incidence of H. pylori infection in the Gezira state. This study indicated that conventional PCR is more sensitive and specific than RUT .The current study recommended that additional studies should be warranted on a larger sample size to study the prevalent H. pylori strains in the Sudan and the combination of at least two methods should be adopted to improve the accuracy of the H. pylori detection.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0e52219b-7e74-4e95-b07b-1b1d7fdaeee6

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

2203436_1.pdf

بيانات النشر

ترجمة العنوان

حدوث وتقييم طرق التشخيص المستخدمة في الكشف عن البكتريا الحلزونية في مستشفى ودمدني التعليمي, ولاية الجزيرة, السودان

ألقاب المؤلفين

[{"name_ar":" Adam Dawoud Abakar Salim ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":" Amany Eltayib Ataelmanan Ahmed","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Mohamed Ahmed Taha ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

The Incidence and Evaluation of Diagnostic Methods used for Detection of Helicobacter Pylori in Wad Madani Teaching Hospital, Gezira State, Sudan (2020)

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

gastrointestinal tract infection -Helicobacter Pylori - diagnosis -Medical Microbiology -Sudan

إشراف وإعداد

الإشراف

Adam Dawoud Abakar Salim , Mohamed Ahmed Taha

الإعداد

Amany Eltayib Ataelmanan Ahmed

الاقتباسات الببليوغرافية