characterization of cell mediated immune responses induced by visceral leishmania major infection

الموضوعات

طب

الملخص

Leishmaniasis are certain diseases caused by some protozoan parasitic species of the genus Leishmania. However, the visceral leishmaniasis (V.L.) is a form of the disease where the pathogens attack some internal organs of human such as liver, spleen and intestine. Knowledge concerning the responses of the human immune system to the parasites are not adequately studied especially in Iraq. The main aim of the present study is to investigate the cell mediated immune (CMI) response of the naturally infected patients attended Al-Dewanyia Children hospital, during Oct. 2006 to Dec. 2007 and were diagnosed as visceral Leishmaniasis. The present study included eighty children of different ages (forty patients, twenty in post healing stage of the same disease and twenty apparently healthy children as control). The expression of certain activation markers such as CD3, CD4, CD8, CD19, CD45RA, CD45RO, CD1a, CD14, CD22, CD44, CD71 and HLA-DR on the peripheral blood lymphocytes (PBL) of the studied children were detected and certain cytokines of the studied groups were estimated. Cellular expression of CD3, CD4, CD8 and CD19 were detected by using direct dual immunofluorescence staining technique while the CD45RA, CD45RO, CD1a, CD22, CD44, CD71, and HLA-DR were detected by using immunocytochemistry staining technique (DAB). The concentrations of each of the interferon-gamma (IFN-γ), tumor necrosis factor (TNF-α), interleukin four (IL-4) and interleukin ten (IL-10) in the serum of the studied groups were determined by sandwich enzyme linked immunosorbent assay (ELISA). The distribution of different lymphocyte subsets were compared in different study groups and the results showed that there was an insignificant difference in CD8, CD19 and slightly increased significant difference in CD4 while there was no significant difference in CD3 in comparison with control group. The results of immunocytochemistry staining showed high significant increase in CD14, CD22, CD44, CD45RA, CD71 and HLA-DR while CD1a showed significant decrease than control group. The results of cytokines determination showed that there was significant increase in IL-4, IL-10, and TNF-α but slightly increase in acute group in comparison to control group and same thing with post healing group. Significant increase in total serum IgG and IgM levels were observed in patients with V.L. compared to control, but we show decrease in total serum IgA level in patient, and the result revealed an increase in complement C3 and C4 serum level in V.L. patient group, where is no significant differences between post healing and control.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0df8dc38-b1fc-4d6a-9964-0f68bba30b17

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1001503_1.pdf

بيانات النشر

ترجمة العنوان

خواص الاستجابة المناعية الخلوية في المرضى المصابين باللشمانيا الاحشائية

ألقاب المؤلفين

[{"name_ar":" نضال عبد المهيمن","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"علي شعلان الأعريجي","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"فاهم محمد دغيم","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

characterization of cell mediated immune responses induced by visceral leishmania major infection

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Immunology – Medical Parasitology – Infectious Diseases (Visceral Leishmaniasis).

إشراف وإعداد

الإشراف

نضال عبد المهيمن, علي شعلان الأعريجي

الإعداد

فاهم محمد دغيم

الاقتباسات الببليوغرافية