Conventional and molecular Characterization of mycobacterial Strains Isolated from Cattle and Humans

الموضوعات

زراعة

الملخص

In the present study, a total of 167 caseated, enlarged andinflammed lymph nodes and tuberculous organs were collectedfrom cattle slaughtered at Slaughterhouses in Khartoum State.Also twenty lyophilized mycobacterial strains previously isolatedfrom both human and animal cases were examined in this studyusing PCR. The aim of this study was to estimate the prevalenceof bovine tuberculosis and to improve identification and to comparebetween identification methods.The purulent materials from the suspected specimens weredecontaminated with 2% NaOH and concentrated bycentrifugation. The smears made from decontaminated purulentmaterials were stained by Ziehl-Neelsen method and culture ontoLowenstein-Jensen medium was done. Results from culture andmicroscopy were compared to a PCR method by amplifying aninsertion element IS6110 which is specific for Mycobacteriumtuberculosis complex.Out of the 167 specimens examined, 35 (20.96%) werefound to have acid-fast bacteria when smears were examinedmicroscopically. Out of the 35 acid-fast bacteria, 22 (62.86%)showed branching filaments and were identified tentatively asMycobacterium farcinogenes. The remaining 13 (37.14%) werebacilli and were identified tentatively as Mycobacterium bovis. Ofthe 167 specimens, 12 (7.19%) showed visible growth onLowenstein-Jensen medium when incubated aerobically at 37°Cbetween 4 and 8 weeks. None of the 22 specimens with branchingfilaments grew using the above decontamination and growthconditions. Ten isolates were nitrate-negative and urease-positive,- 8 -and were thus identified as M. bovis, whereas the remaining towstrains were nitrate-positive and urease-negative but one wasurease-positive.Polymerase chain reaction (PCR) assay for the rapiddiagnosis of M. tuberculosis complex (M. tuberculosis, M. bovis, M.microti and M. africanum) was done using oligonucleotide primersTB41 and TB42 to amplify fragments of the IS6110, an insertionsequence specific for M. tuberculosis complex. Of the 12 grownculture, ten (83.3%) gave PCR product for IS6110 on agarose gelelectrophoresis, which confirmed their identity as M. tuberculosiscomplex.Ten isolates were already found nitrate negative and thuswere identified as M. bovis, none of the isolates were found to beM. tuberculosis when using this test combination.In conclusion, IS6110 amplification, urease and nitrate testsprovide a species identity to M. bovis. The results of the abovestudy is as follows: of the 167 caseated and purulent specimensexamined 10 (5.99%) were M. bovis, 2 (1.2%) weremycobacterium other than tuberculosis (MOTT), 22 (13.17%) weretentatively identified as M. farcinogenes, 23 (13.77%) were acidfastbacteria (AFB) that failed to grow on L.J medium and 132(79.04%) were caseous infections caused by non-acid-fastbacteria.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0ded1491-ed17-498b-8bcd-34f3cc580dc0

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1639797_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Manal Hassan Salih","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Mohamed El-Amin Hamid","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Conventional and molecular Characterization of mycobacterial Strains Isolated from Cattle and Humans

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Mohamed El-Amin Hamid

الإعداد

Manal Hassan Salih

الاقتباسات الببليوغرافية