استخلاص البيتاغلوكان من خميرة الخباز بطرائق مختلفة ودراسة صفاته الوظيفية

الموضوعات

زراعة

روابط وملفات

التعريف والنوع

رقم الوثيقة

0dd6cb97-b6aa-4448-add8-799dcc33ae3f

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1965675_1.pdf

بيانات النشر

ترجمة العنوان

Extraction of Beta Glucan from Baker's yeast using Different Methods and Studying its Functional Properties

ألقاب المؤلفين

[{"name_ar":"كنانة ممدوح عليكو ","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"لينة الأمير ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"محمد محمد ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

استخلاص البيتاغلوكان من خميرة الخباز بطرائق مختلفة ودراسة صفاته الوظيفية

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

Betaglucan of the cell wall of baker's yeast Saccharomyces cerevisiae was extracted from the yeast cell wall that was analysed using three methods ;autolysis under controlled conditions at (pH 5, temperature 50 °C and 48 hours incubation), autolysis at uncontrolled conditions by incubating at room temperature for 13 days and the third method was plasmolysis using 5%sodium chloride salt. The percentage of the cell wall produced by the yeast extracts obtained by the three methods ranged between 14-39%. Betaglucan of crude cell wall was extracted by the alkaline –warm up method under different conditions ( time, concentration of alkali and proportion of the wall of yeast to alkaline). The percentage of betaglucan was estimated depending on dry weight of cell wall using phenol -sulfuric acid method . The treatment using 1N sodium hydroxide and a ratio of 1 g yeast / 5 ml alkaline for 2 hours gave the highest percentage of crude β-glucan of 52.65% using the method of Autolysis under controlled conditions, while the two other methods of Autolysis under uncontrolled conditions and plasmolysis reached percentages of 40.66,44.33%,respectively. Autolysis under controlled and that of uncontrolled conditions overcame the method of plasmolysis in their average values of total carbohydrates concentration (crude β-glucan).)6-1(β Glucan was separated from )3 -1(β Glucan using phosphoric acid-ethanol treatment, so that the treatment 2/2/1:2 gave the highest rate reaching 4.2g using the plasmolysis method, then a partial purification process for crude beta glucan was conducted, by treatment with phosphoric acid and high temperature to precipitate the protein . Percentages of protein during the extraction and purification steps were estimated by different analysis methods, they ranged between 0.68-1.31g/100g dry weight of cell wall, percentages of glycogen were also estimated using different analysis methods, they ranged between 41.80–52.42g/100g dry weight. Proportion of β-glucan partially purified was estimated using high-performance liquid chromatography HPLC of enzymatically hydrolysed samples treated with β-(1→3) -D-Glucanase, autolysis under controlled conditions reached the the highest value reaching 29.5% followed by the method of autolysis under uncontrolled conditions and plasmolysis with values of 21.4-12.8% respectively. Highest susceptibility to bind fat and water where reported by controlled conditions method and reached 242 ml/100g, 231ml/100g respectively. Pasteurization process influenced the ability to bind fat negatively leading to a decline in the ratio up to 231 ml / 100,whereas acidification process influenced the ability to bind water positively leading to a high percentage reached 748.22 ml / 100 g. On the other hand, beta glucan offered high ability to make foam reaching 8.5,8.5,9.5 ml in plasmolysis, autolysis under uncontrolled and under controlled conditions respectively, also acidification treatment led to reduction in the stability of the foam and its formation. Heating and cooling treatments influenced the stability of foam and led to its significant decline .

إشراف وإعداد

الإشراف

لينة الأمير , محمد محمد

الإعداد

كنانة ممدوح عليكو

الاقتباسات الببليوغرافية