Isolement de souches bactériennes potentiellement productrices de lipases alcalines, à partir du beurre cru et du beurre fermenté «Dhan»

الموضوعات

علوم تطبيقية وتكنولوجية

روابط وملفات

التعريف والنوع

رقم الوثيقة

0da3709a-289d-4743-97fc-da10c3d62dac

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

789419_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Farida Bekhouche","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Nora Belhoula","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

French

المصدر والدورية

اسم المصدر

Isolement de souches bactériennes potentiellement productrices de lipases alcalines, à partir du beurre cru et du beurre fermenté «Dhan»

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

Considerable interest is carried currently for lipase, mainly to industrial sectors, aswell as for the lipolytic flora of the local traditional products. In this context, settles the goalof our work on isolation of lipolytic strains from raw butter and smen, which are twotraditional Algerian dairy products. As well as the screening of the best bacterial isolatesproducing lipases. To achieve our goals, six samples of varied origin were used for thephysicochemical analysis and the isolation of lipolytic bacterial strains. We used severalmedia, all these are supplemented with Of egg yolk or olive oil and / or Tween 80. The strains obtained are classified for its activities on agar medium and then grown on Liquid media.Titration is the method used to estimate free fatty acids or lipase activity. Physicochemicalcharacterization results showed that there was a significant difference between the measured parameters of butter and smen samples. Screening yielded to obtaining of several lipolytic isolates from raw materials rich in fat, namely : raw butter and smen. The test of the lipolytic activity has allowed selecting Forty-nine bacterial strains which are approximated on the basis of the phenetic results of six types : 49% (24) are Gram-positive, Branched filamentous, catalase positive, asporulated and immobile ; 31% (15) are Gram-positive, cocci, catalase positive, asporulated, immobile and degrade mannitol ; 10% (5) are Gram-positive, rod, catalase positive, asporulated and immobile ; 4% (2) are Gram-positive, cocci, Catalase positive, asporulated, immobile, does not degrade mannitol and have a yellow color ; 4% (2) are Gram-positive, rod, catalase positive, sporulated and mobile and 2% (1) are Gramnegative, cocci, catalase positive, asporulated and immobile. The test of production of lipase on liquid medium containing inducers of the production of the enzyme (Tween 80, olive oil with or without Tween 80) yielded to the selection of eight strains (SG (5, 8, 9, 14, 25, 26 ) ; BG (32) ; SS (46)), potentially producing true lipases independently of the nature of the lipid source. This finding reveals the degree of adaptation of these strains to various media, giving it various biotechnological applications.

إشراف وإعداد

الإشراف

Farida Bekhouche

الإعداد

Nora Belhoula

الاقتباسات الببليوغرافية