Antibacterial Activity of Nigella sativa extract against selected bacterial clinical isolates from Khartoum State

الموضوعات

علوم بحتة وطبيعية

الملخص

An alarming increase in bacterial strains resistant to existing antibacterialagents demands a renewed effort to seek agents effective againstpathogenic bacteria resistant to antibacterials.The aim of this study was to study the antibacterial activity of differentconcentrations of petroleum ether and methanol extracts of medicinalplant Nigella sativa (seeds) using cup-plate agar diffusion method onslected clinical isolates of bacteria.The study was conducted in Khartoum state, Sudan, during the periodfrom June to November 2015.One hundred forty four samples were collected form urine, wound andstool, 103/144 (72%) showed bacterial growth, from which nine types ofpathogenic bacteria were isolated and identified using Gram stain,biochemical reactions and tested for their susceptibility to a number ofantibiotics. Those were resistant to three or more antibiotics were testedfor their sensitivity to Nigella sativa extracts. The tested isolates included,Klebsiella pneumoniae 32(31%), Escherichia coli 22(21%),Staphylococcus aureus 21(20%), Proteus mirabilis 11(11%) andPseudomonas aeruginosa 9(9%).The petroleum ether and methanol extract of Nigella sativa were screenedfor their antibacterial activity against standard and clinical isolates.Petroleum ether extract of Nigella sativa showed pronounced dosedependant antibacterial activity on standard strains and clinical isolates,while methanolic extract showed no activity.Petroleum ether extract of Nigella sativa showed antibacterial activityagainst standard and clinical isolates of S.aureus, K.pneumoniae andE.coli, while only standard strains of P. mirabilis and Ps. aeruginosawere sensitive to it.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0d085669-4819-4109-a808-5385e242406a

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

21349_1.pdf

بيانات النشر

ترجمة العنوان

نشاط مستخلص الحبة السوداء علي البكتریا المعزولة من عینات طبیة بولایة الخرطوم

ألقاب المؤلفين

[{"name_ar":" أحمد إبراهيم هاشم","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"ماهي الجاسم علي محمد","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Antibacterial Activity of Nigella sativa extract against selected bacterial clinical isolates from Khartoum State

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

أحمد إبراهيم هاشم

الإعداد

ماهي الجاسم علي محمد

الاقتباسات الببليوغرافية