Micropropagation of Acacia Seyal Var. Seyal Del. Using Single Nodle Explants

الموضوعات

علوم بيئية

الملخص

A.seyal var. seyal (Talh tree) is considered as an important multipurposetree species in Sudan, and there is a great need for developing in vitropropagation methods for clonal propagation of elite cultivars.The aim of this study is to develop a direct micropropagation of A. seyalplantlets and test the effect of basal salt mix of MS medium and somegrowth regulators on shoot growth and root formation.Single node explants was obtained from mature tree of A. seyal and werecultured on Murasige and Skoog medium (1962). MS strengths, sucroseand glucose as source of carbohydrates, inositol, MS vitamins andmodified White vitamins (1939), adenine sulfate, activated charcoal andsilver nitrate are tested as experiments of basal MS media. The growthregulators, cytokinins and auxins are tested singly.The results show that 1.0x and 0.5x (full and half) strength of MS saltmix medium are the best basal salts for A. seyal, 2-3% w/v of sucrose;100mg/l inositol, asingle dose (1.0x) of MS vitamins; 40.0mg/l adeninesulfate; 2.0g/l of active charcoal and 1.0 and 5.0mg/l silver nitratearequite suitable for propagation of Talh trees.The addition of 1.0 or 1.5mg/l BA, 1.5 or 2.0mg/l kinetin, 1.5 or 2.0mg/lIBA, 1.5 or 2.0mg/l NAA are suitable concentrations for culture of singlenode explants of Talh trees.Excised shoot from in vitro explants were used for rooting. Differenttypes of MS strength were tested with different concentration of IBA.Rooting ability was achieved on ¼ MS medium with 0.5mg/l IBA(rooting 30%).

روابط وملفات

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التعريف والنوع

رقم الوثيقة: 0c95f7ae-fd4b-40de-9c42-5a435e5757f8
رقم العقد: 0
نوع الوسائط: Crawler
نوع المحتوى: الرسائل العلمية
صيغة المصدر: رسائل ماجيستير
نوع الملف: pdf text
أسماء الملفات: 1637912_1.pdf

بيانات النشر

ألقاب المؤلفين: [{"name_ar":"Dr. ESAM IBRAHIM WARRAG","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Esam Ibrahim Warrag","title_ar":"تصنيف","title_en":"Classification"},{"name_ar":"Ghada Salih Altayeb","title_ar":"اعداد","title_en":"Preparation"}]
اللغة: English

المصدر والدورية

اسم المصدر: Micropropagation of Acacia Seyal Var. Seyal Del. Using Single Nodle Explants

المحتوى والصفحات

عدد الصفحات: 0

إشراف وإعداد

الإشراف: Dr. ESAM IBRAHIM WARRAG
الإعداد: Ghada Salih Altayeb

الاقتباسات الببليوغرافية

APA

Dr. ESAM IBRAHIM WARRAG،Esam Ibrahim Warrag و Ghada Salih Altayeb. (2004). Micropropagation of Acacia Seyal Var. Seyal Del. Using Single Nodle Explants. أطروحة(رسائل ماجيستير). كلية الغابات-جامعة الخرطوم. السودان.

MLA

Dr. ESAM IBRAHIM WARRAG،Esam Ibrahim Warrag و Ghada Salih Altayeb. Micropropagation of Acacia Seyal Var. Seyal Del. Using Single Nodle Explants. 2004. كلية الغابات-جامعة الخرطوم، رسائل ماجيستير.