Studies on Q Fever in Farm Animals in Kingdom of Saudi Arabia

الموضوعات

طب

الملخص

The serological prevalence of Coxiella burnetii in domestic livestock inSaudi Arabia was studied using two serological tests: indirect enzymelinked Immunosorbent assay (ELISA) as the main test and indirectimmunofluorescence assay (IFA) as a confirmatory test and for.comparison with ELISAA total of 1970 farm animals of both sexes were tested serologically todetermine the prevalence of C. burnetii specific IgG antibodies usingindirect ELISA. The samples were collected from 489 camel, 428 cattle,630 sheep and 423 goats. The animals were broadly divided into youngand adult animals. All of them were clinically normal when sampled and.none of the adult females was pregnant while some were lactatingA total of 605 animals had anti-C. burnetii IgG antibodies in their sera,giving an overall serological prevalence of Q fever of 30.71% with amean ELISA titre (S/P ratio or O.D.%) of 103.03%. These results indicatethat C. burnetii is common in all species a of farm animals in SaudiArabia. Camels showed the highest proportion of Q fever (C. burnetii)positive sera among all the species tested, with an overall prevalence of51.53%. The second highest serological prevalence was recorded in goats(34.04%), followed by cattle (30.61%) and the least in sheep (12.38%). Inall species, the serological prevalence of anti-C. burnetii antibodies wassignificantly higher in adult compared to young animals (p<0.0001).Females animals tended to be more commonly affected than males;however, statistical analysis revealed non-significant inter-sex difference(p= 0.5847). Antibodies against C. burnetii in domestic livestock werealso investigated using ELISA assay in 285 defatted milk samplesobtained from 48 she-camels, 90 cows, 60 ewes and 87 does. Milksamples from 30 camels (62.5%), 30 cows (33.3%), 24 goats (27.6%) andii3 ewes (5%) were positive for anti-C. burnetii antibodies. Serum samplesfrom the same animals were simultaneously tested by ELISA. Of these,32 camels (66.66%), 38 cows (42.2), 13 goats (14.9%) and 4 ewes(6.67%) were positive for anti-C. burnetii antibodies. Statistical analysisshow a significant correlation between ELISA results in milk and serum.Serum samples from a total of 307 animals, comprising 92 camels, 72cows, 72 sheep and 71 goats, were also subjected simultaneously toindirect immuno-fluorescence (IFA) and ELISA assays. A statisticallysignificant correlation was found between the serological prevalence of Qfever as determined by these two assays. Using ELISA as a referenceserological test, statistical analysis showed that both the sensitivity andspecificity of IFA assays were good, indicating that either ELISA or IFAcan be used for screening Q fever in farm animals or as confirmatory teststo one another. The shedding of C. burnetii by serologically positiveanimals was investigated by the polymerase chain reaction (PCR), usingprimers that amplify the repetitive transposon-like region of C. burnetii.The study was conducted on 82 whole blood, 72 milk, 29 faecal and 21urine samples collected from camels. In addition, 29 milk samples and 7whole blood samples from cattle, 38 whole blood, 29 milk and 20 faecalsamples from goats and 22 blood samples from sheep were available for.PCR analysisOut of a total of 149 whole blood samples collected from these differentanimal species, 13 samples (15.85%) from camels and 2 samples (5.6%)from goats showed positive amplification for C. burnetii DNA while all22 sheep and 7 bovine blood samples were negative. Out of 144 milksamples collected from camels, cattle and goats, 5 samples (6.49%) fromcamels, 11 samples (28.94%) from cows and 0 samples from goats werepositive for C. burnetii DNA. In addition, faecal samples collected from29 camels and 20 goats revealed positive PCR products from 8 (27.59%)iiiand 12 (60%) samples, respectively. C. burnetii DNA was alsodemonstrated in 5 (23.81%) out of the 21 urine samples collected fromcamels. All sampled subjected to PCR analysis were from serologicallypositive animals with the exception of urine samples which werecollected from slaughtered camels that were not serologically tested foranti-C. burnetii antibodies. Serum samples from known Q fever-positiveand known Q-fever negative animals were used to study the possibleeffects of Q fever on various biochemical and electrolyte parameters. Atotal of 281 serum samples were collected from camels, sheep, goats andcattle. In all species, no significant differences were found betweenQ-fever positive and Q-fever negative animals. However, a fewintra-specific differences existed within each species. The effect of Qfever was also investigated in the levels of anti-oxidant enzymes, namelythiobarbituric acid reactive substances (TBARS) and reduced glutathione(GLUTH). TBARS level was determined in 239 known Q fever-positiveand Q fever-negative animals while GLUTH level was determined in 188known Q fever-positive and Q fever-negative animals. No significantdifferences in TBARS levels were found between Q fever-positive and Qfever negative animals in samples collected from each of goats, camelsand sheep. However, the GLUTH level was found to be significantlyreduced (p<0.002) in Q fever-positive camels as compared to Q fevernegative camels. This enzyme is found in the cytoplasm of almost allmammalian cells, and a reduction in its activity could indicate somedegree of cellular damage. However, further studies are needed to verify.this aspectThis study constitutes the first record of C. burnetii in cattle, sheep andgoats in Saudi Arabia and the second, and more detailed, study on camels.in the country

روابط وملفات

التعريف والنوع

رقم الوثيقة

0c883353-6a9a-4580-a841-b08aaf3a2dfa

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

31754_1.pdf

بيانات النشر

ترجمة العنوان

دراسات عن الحمى المجهولة في حيوانات المزرعة في المملكة العربية السعودية

ألقاب المؤلفين

[{"name_ar":" أمل عمر بخيت","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":" محمد الشيخ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"عبد الرحمن جار النبي عبد الرحمن","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"منصور فارس حسين","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Studies on Q Fever in Farm Animals in Kingdom of Saudi Arabia

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

أمل عمر بخيت, محمد الشيخ, منصور فارس حسين

الإعداد

عبد الرحمن جار النبي عبد الرحمن

الاقتباسات الببليوغرافية