Phenotypic and Proteomic characterization of Clinical Candida albicans isolates resistant to Fluconazole

الموضوعات

علوم بحتة وطبيعية

الملخص

The opportunistic fungal pathogen Candida albicans is a major causative agent of death in immunocompromised individuals. The azole fluconazole is the first line of defense in hospital treatment. Azoles function by inhibiting Erg11 an enzyme involved in the synthesis of ergosterol, the main sterol found in fungi. Resistance to azoles is on the increase worldwide including in Lebanon. The purpose of the current study is to determine pathogenicity attributes of azole resistant isolates from Lebanese hospital patients. Six isolates-3 resistant and 3 sensitive-underwent a battery of phenotypic tests related to virulence. In addition, the cell wall proteome of all isolates was isolated and underwent profiling by MALDI TOF TOF mass spectrometry to detect different proteins in resistant versus sensitive isolates, and differential presence in response to fluconazole exposure. Our results showed that resistant isolates had increase ergosterol content, and a slight increase in chitin deposition leading to resistance, but were on average attenuated in virulence compared with sensitive isolates. In addition, proteomic profiling revealed the presence of efflux pumps such as Cdr1, and Cdr2 exclusively in resistant isolates, an additional mechanism of resistance, and virulent isolates expressed more virulence genes as opposed to virulence attenuates isolates that did not express any.In conclusion, our study suggests several mechanisms of antifungal drug resistance in C. albicans Lebanese hospital isolates.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0c2baa4f-9004-4775-b9f7-77b7da3e5c48

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2212792_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Roy Khalaf","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Geovanni Varoujan Geukgeuzian","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Phenotypic and Proteomic characterization of Clinical Candida albicans isolates resistant to Fluconazole

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Fluconazole C.albicans pathogenicity attributes MALDI Antifungal resistance

إشراف وإعداد

الإشراف

Roy Khalaf

الإعداد

Geovanni Varoujan Geukgeuzian

الاقتباسات الببليوغرافية