Acute Toxicity of Crude Ethanol Extract of Leptadenia pyrotechnica Forsk Against Culex quinquefasciatus Say Larvae

الموضوعات

علوم بحتة وطبيعية

الملخص

This study was carried out to examine the acute toxicity of the crude ethanol extract of the branches of Leptadenia pyrotechnica (Forsk.), called Markh in Arabic, against laboratory reared late third instar larvae of Culex quinquefasciatus Say. The main purpose of this study was to find easily obtainable, cheap, safe and environment friendly control substances. The plant material was obtained from Um Dam Haj Ahmed Locality in Northern Kordofan State , in October 2011, during the flowering stage. The plant material was extracted by absolute ethanol using a Sohxlet extractor. The extract was allowed to condense in a rotary evaporator into a nearly paste form and left open at room temperature until the ethanol has evaporated and the extract changed into a powder form. Following World Health Organization recommendations, a bioassay of five concentrations [350,500,650,800 and 950 part per million (ppm)] were carried out to give a complete mortality from 100W22; to 0+% in mosquito larvae in addition to the control. The concentrations were prepared by adding 1.9, 1.6, 1.3, 1.00 and 0.70 ml of the stock solution of plant extract, using micropipettes, to 200 cm³ of dechlorinated tap water. The control was set up with 1.9 cm³ of absolute ethanol and 200 cm³ of dechlorinated tap water. Every concentration was tested with a total of 100 late third instar laboratory reared larvae repeated as four replicates, each with 25 larvae. Mortality was recorded throughout the exposure period and observed to increase with concentration. No mortality of larvae was observed in the control. The twenty four hours LC50 (lethal concentration fifty %) and LC90 (lethal concentration ninety %) and the forty eight hours LC50 and LC90 were calculated using probit double transformation regression analysis. The twenty four hours LC50 was 826 ppm and the LC90 was 2070 ppm. The forty eight hours LC50 was 601 PPM and the LC90 was 1153 ppm. The results of this study prove that this plant extract contained mosquito larvicidal compounds the activity of which increased with the time of exposure. The effect of this extract on non-target organisms have not evaluated in this study. The findings of this work were discussed on the light of related published work.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0c0de15f-3fe9-4406-834a-11edb939f7a0

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf image

أسماء الملفات

0c0de15f-3fe9-4406-834a-11edb939f7a0_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Ahmed Ashraf Fadul Ali","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Tigani M. H. Allam","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Acute Toxicity of Crude Ethanol Extract of Leptadenia pyrotechnica Forsk Against Culex quinquefasciatus Say Larvae

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Tigani M. H. Allam

الإعداد

Ahmed Ashraf Fadul Ali

الاقتباسات الببليوغرافية