تحديد بعض المؤشرات الجزيئية المرتبطة بمورثات المقاومة لصدأ الأوراق في القمح القاسي

الموضوعات

علوم تطبيقية وتكنولوجية

روابط وملفات

التعريف والنوع

رقم الوثيقة

0bb1ef13-0b2b-46f9-b64e-9a43473a8621

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1960306_1.pdf

بيانات النشر

ترجمة العنوان

Identification of Some Molecular Markers linked to Leaf Rust Resistance Genes in Durum Wheat

ألقاب المؤلفين

[{"name_ar":"رهام أحمد أبو الكنج","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"غنوة لبابيدي ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"نعيم الحسين ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

تحديد بعض المؤشرات الجزيئية المرتبطة بمورثات المقاومة لصدأ الأوراق في القمح القاسي

المحتوى والصفحات

عدد الصفحات

132

ترجمة الملخص

Leaf rust, caused by Puccinia triticina is a widespread disease of wheat causing substantial yield losses in wheat producing regions around the worldIts importance has increased in Syria during the last decade, due to an increases in wheat irrigated area as well as the susceptibility of durum wheat to the disease. This study aimed at:1.Evaluation the reaction ofdurum wheatgenotypes against the natural community of leaf rust in the seedlingstage.2 .The genetic diversity analysis of the genotypes using simple repeat sequence (SSR) technique.3. Identification ofleaf rustresistance genes in durum wheat genotypes.The research was carried out in the Biotechnology Laboratory of the Aleppo Research Center and Molecular marker Lab.of the International Center for Agricultural Research in the Dry Areas (ICARDA) at the Faculty of Agriculture, University of Aleppo, during 2016-2017.Thereaction of 35 genotypes and 9 released cultivarsin Syria of durum wheat was evaluated against the natural community of leaf rust in the seedlingstage, genetic diversity of allthesegenotypes was studied,DNA was extracted from all genotypes with CTAB method, and determined its quality, purity, and quantity.Fourteen pairs of Simple Sequence Repeats(SSR)primerswere used, the dendrogramwith aUPGMA methodwas drawnand the genetic similarities between the genotypes were studied using Jaccard coefficient.The polymorphic information content (PIC) was calculated polymorphic frequency, and the structure of the wheatgenotypes was studied usingthe Structure 2.3.4 software.Twenty pairs of specialized SSR primers were used to detect 20 genes ofleaf rust resistance geneswithin the studied genotypes compared with the differentialvarietieswhichused as a positive control.The results of the morphological study showed variation in the reaction of the genotypes to the natural community of leaf rustin the seedlingstage.Based on the bioassay scale; the genotypes were divided into four main groups: resistant (0-1), moderately resistant (2), moderately susceptible (3) and susceptible (4); which were estimated with 34%, 32%, 11% and 23%, respectively. The results of theDNAextractionshowed that theconcentration ranged between133.9-1048 ng/μl.The ratio OD260/OD280ranged between 1.75 -2.09interferred good purity.DNA quality at 1% agarose gel showed that,all DNA samples with high molecular weight and free from smear.The results of the genetic diversity showed 13 SSRprimersto detect the polymorphism of the tested genotypes,they gave 160 alleles with an average of 12.3 alleles per locus,their length ranges between 100-2000bp,the percentage of polymorphism varied between 33.3-100%, andthe polymorphic information content (PIC)ranged between 0.14-0.37, the wms70, wms666,wms642,wms408primers gave the best resultsbased on the number of alleles produced and the PIC value. The genetic similarity between the studied genotypesranged from 0.21 to 0.9. The cluster analysis also showed the distribution of the studied genotypesin two main clusters according to their genetic origin, as confirmed by the results obtained through structure analysis.The results of the molecular detection of the resistance genes showed that the studied genotypes possessedthe following resistance genes: Lr14, Lr17, Lr19, Lr24, Lr25, Lr27, Lr28, Lr29, Lr34, Lr37, Lr39, Lr46 and Lr48with differentproportions.Six genes namesLr9, Lr20, Lr26, Lr35, Lr47 andLr68weren‘tavailable.Eight genotypes were identified as a model forlong-term resistance, each possessedmore than one majorand minorresistantgenes. Fifteengenotypes possessedat leastone of slow rust genes Lr34andLr46. Both genotypes are a good genetic source of leaf rustresistantand are recommended forwheatbreeding programs.

إشراف وإعداد

الإشراف

غنوة لبابيدي , نعيم الحسين

الإعداد

رهام أحمد أبو الكنج

الاقتباسات الببليوغرافية