Bacterial Contamination of Multi-Dose Veterinary Medication Vials in Dairy Cattle and Poultry Farms in Khartoum State

الموضوعات

علوم بحتة وطبيعية

الملخص

The objective of this study was to evaluate bacterial contamination in multidose veterinary medication vials in dairy and poultry farms in KhartoumState. A total of 324 samples of 22 veterinary medications were collectedfrom 35 dairy and poultry farms. The medication types included injectable(antibiotic and non-antibiotic) and non-injectable drugs. Samples frominjectable drugs constituted 96% of total number of samples. Samples werecultured on Blood Agar and MacConkey’s Agar plates and incubatedaerobically at 37oC for 48 hours. The antibiotic samples were cultured afterbeing centrifuged and washed with sterile normal saline for three times toobtain antibiotic-free culturable sediment. Forty-one isolates from 36samples of 12 medications were recovered; with a contamination prevalenceof 11.1%. No isolate was recovered from antibiotic samples (0/225). Thecontamination prevalence in non-antibiotic samples was 36.4% (36/99); innon-antibiotic injectables was 31.4% (27/86) and in non-sterile medicationswas 69.2% (9/13). The isolates weredifferentiated according to theirmicroscopic, culturing and biochemical properties to the following bacterialspecies: Pseudomonas aeruginosa(3.1% of total samples), Bacillus cereus(2.5%), Staphylococcus aureus(2.5%), Micrococcus varians(1.5%),Micrococcus luteus(1.2%), Staphylococcus haemolyticus(0.6%), Neisseriamucosa(0.6%), Staphylococcus epidermidis (0.3%) and Staphylococcusauricularis(0.3%). Pseudomonas aeruginosa, Staphylococcus aureusandBacillus cereuswere isolated from both dairy and poultry farms; whereasother species were obtained only from dairy farms. The data collected withsamples (storage conditions,opening and expiry dates of vials, sterility of11used syringes and others) indicated that dealing with multi-dose vials ismuch violated.To conclude, the bacterial contamination prevalence in non-antibioticmedications (parenteral and non-parenteral) was found to be very high, andserious due to isolation of known potentially pathogenicbacteria, whichmight lead to spread of serious systemic infections if injected. Violatedstorage conditions, administrating method and expiry and opening dates ofmulti-dose medication vials were observed in the visited farms and couldjustify the result.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0aadaf74-cbc8-4a8d-af6b-88731c7fdd12

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1637914_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Elhassan M. A. Saeed","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Hytham Eltaj Yousif Omer","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Bacterial Contamination of Multi-Dose Veterinary Medication Vials in Dairy Cattle and Poultry Farms in Khartoum State

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Elhassan M. A. Saeed

الإعداد

Hytham Eltaj Yousif Omer

الاقتباسات الببليوغرافية