Caractérisation de la production de la vitamine B12 chez les bactéries probiotiques

الموضوعات

علوم بحتة وطبيعية

الملخص

In the present study, eleven beneficial isolates from faeces of exclusively breastfedinfants and seven more from human milk origins were explored for their vitamin B12productivity. For this purpose, their safety and antioxidant activity were estimated in vitro bymeasuring the DPPH free radical scavenging activity and the FRAP iron reducing capacity.Their antimicrobial activity against selected pathogenic strains was studied by the sinkmethod. The results found reflected DPPH free radical scavenging activities of our probioticisolates in the range of 16 to 32.1%. However, there is a disparity in this antioxidant capacitybetween isolates from human milk and those from infant faeces. By the FRAP method, thedata were not changed, the antioxidant activities were lower than 1 in the lactic strains SL3,SL7 and SL9 and about 1.34% in the SL4 isolate which marks its superiority. The intestinalstrains showed antioxidant activities ranging from 1.22-1.48%; excepted SS10 and SS11whose values were lower than 1%. Supernatants obtained from the 11 faecal isolates showedvarying degrees of inhibitory activities. The most interesting faecal isolate was SS8 where thecalculated inhibition diameters were all greater than or equal to 10 mm, excepted thoseagainst the two pathogens S. aureus and Klebsiella pneumoniae where the inhibition zoneswere 8 to 9 mm. The supernatants obtained from the seven lactic isolates of breast milkorigin also showed varying degrees of inhibitory activity and would appear to be moreinteresting compared to those calculated for the beneficial faecal isolates. All isolates wereable to secret cobalamin. This productivity was found to be similar at both λ=240 and 245 nmwavelengths. It would be interesting to incorporate these strains in the composition ofprobiotic foods in order to enrich them in situ with B12 vitamin.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0a920b0c-d49f-42ab-9033-665645c4fbf9

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

ماستر (LMD)

نوع الملف

pdf text

أسماء الملفات

2145004_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"H Ziar","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"سامية دخيشي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

French

المصدر والدورية

اسم المصدر

Caractérisation de la production de la vitamine B12 chez les bactéries probiotiques

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

beneficial isolate, human milk, infant faeces, antioxidant activity, antimicrobial activity, cobalamin.

إشراف وإعداد

الإشراف

H Ziar

الإعداد

سامية دخيشي

الاقتباسات الببليوغرافية