Antimicrobial activity of a Number of Streptomyces species

الموضوعات

طب

الملخص

Streptomycesa gram-positive filamentous bacteria, that is widely distributed in most soilsand decaying vegetation. Most Streptomycetesreproducebyspores anddistinct for their earthy odor that result fromtheproduction of a volatileterpene called geosminwhichis one of the most famous metabolites that are produced by this genus. The discovery of streptothricin in 1942was the starting point of history of antibiotics derived from Streptomyces, and thenthe discovery of streptomycin two years later, after which scientists intensified research’s for antibiotics within the genus. Today, a large number ofantibiotics are produced bythe genus Streptomyces, producing antibacterial, antifungal, and antiparasitic drugs, and also a large numberof other bioactive compounds, such as immunosuppressants. It is thought that the productions of the bioactive compounds produced by Streptomyces areinitiated during the time coinciding with the aerial hyphal formation from the substrate mycelium. Streptomycinand oleandomycinare examples of antimicrobial compounds against tuberculosisthat are produced by Streptomycesgriseus and Streptomyces antibioticusrespectively. FK506an immunosuppressant is another bioactive compound that is produced by Streptomyces tsukubaensis, while avermectin, bleomycin, and daunomycinare antitumor compoundsthat are produced by StreptomycesavermitilisStreptomyces verticillusandStreptomyces peuceticusrespectively.In this research, Streptomycespratensis QUBC97 was isolated on CCG medium and identified by molecular methods. The isolate produced an antibacterial agent when grown in CCG broth that showed tow distinguishable compounds from fresh or old cultures. The freshly produced compound was extracted in n-butanol and showed an Rfvalue of 0.86 when developed on silica gel TLC plates in n-butanol saturated with 1.7% ammonium acetate. The compound absorbed visible light at 426 and 440 nm. Putative identification of HPLC purified and concentrated compound using mass spectroscopy suggested the compound to be methoxy, 1-phenyl-, oxime. This work is a step in the direction of developing our capacity in detection, isolation, and industrialization of antimicrobial agents.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0a8a778e-fdc5-4454-beae-c9e81a96d72d

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1696589_1.pdf

بيانات النشر

ترجمة العنوان

نشاط المضادات الحيوية لعدد من أنواع الستريبتومايسز

ألقاب المؤلفين

[{"name_ar":"ابتهال محمود ربيع عياد","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"سمير البرغوثي","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Antimicrobial activity of a Number of Streptomyces species

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

سمير البرغوثي

الإعداد

ابتهال محمود ربيع عياد

الاقتباسات الببليوغرافية