Constituants chimiques, propriétés cytotoxiques, antifongiques et antibactériennes de l’extrait chloroforme de Centaurea diluta Ait. subsp. algeriensis (Coss. & Dur.) Maire

الموضوعات

علوم بحتة وطبيعية

روابط وملفات

التعريف والنوع

رقم الوثيقة

0a89812e-cd59-4162-927c-57874b4cffaa

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf image

أسماء الملفات

0a89812e-cd59-4162-927c-57874b4cffaa_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Fadila Benayache","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Hanène Zater","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

French

المصدر والدورية

اسم المصدر

Constituants chimiques, propriétés cytotoxiques, antifongiques et antibactériennes de l’extrait chloroforme de Centaurea diluta Ait. subsp. algeriensis (Coss. & Dur.) Maire

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

This work is apart of our researchunit program relating to the phytochemicaland biological studies on the plants of the genus Centaurea (Asteraceae). The speciesof this genus are potential reserves of bioactive secondary metabolites.The present work concerned initially the in vitro evaluation of the cytotoxic,antifungal and direct and indirect antimicrobial activities of the chloroform soluble part ofthe hydro-alcoholic extract of the aerial parts of Centaurea diluta Ait. subsp. algeriensis(Coss. & Dur.) Maire, an endemic species to Algeria and Morocco. According to an exhaustivebibliographical research carried out on Centaurea species, no phytochemical nor biological reportis available on this subspecies so far; which constitutes one of our selection criteria for this plant.Inactive against 7 tested microorganisms (plant pathogens) fungi, bacteria and yeast, andalso inactive in the direct antibacterial test, this extract displayed significant cytotoxic effects oncancer cells (the A549 non-small-cell lung carcinoma, the MCF7 breast adenocarcinoma and theU373 glioblastoma). In indirect antibacterial tests, the CHCl3 extract was able to potentiate theeffect of -lactam antibiotics on methicillin-resistant Staphylococcus aureus (MRSA), reducingthe minimal inhibitory concentrations (MICs) by a factor of 2-32-fold. No synergy was foundbetween the extract and streptomycin. Biological data allowed to guide the chemical compositionof the extract by fractionation, separation and purification on silica gel 60 (CC and TLC).This study led to the isolation of nine components in a pure state and the identification offive of them, by the combination of several spectroscopic analyses. These products weretwo phenolic compounds (vanillin 1, paridol 3), a lignane [(-arctigenin 2] and two flavonoidaglycones (eupatilin 4 and jaceosidin 5). All the isolated compounds are described for the firsttime for this species. The 5 identified compounds were evaluated for cytotoxic and antimicrobialactivities. Moderate cytotoxic effects were observed on the six tested cancer cell lines (A549,MCF7, U373, Hs683 human glioma, PC3 human prostate and B16-F10 murine melanoma)for both the flavonoid aglycones and the lignane with IC50s in the range 28 μM to 40 μM.In the direct antibacterial test by TLCbioautography, among the five isolatedcompounds, only jaseosidin 5 showed a moderate antimicrobial activity but thisactivity was relatively weak on the positive and negative Gram bacteria (MIC of 200 μg/mLon MRSA C98506, MRSA C100459, MRSA ATCC33591, MSSA ATCC6538, E. coliATCC25922).

إشراف وإعداد

الإشراف

Fadila Benayache

الإعداد

Hanène Zater

الاقتباسات الببليوغرافية