Surface Coating of Carbon Nanofiber by Mesoporous Silica: a Promising Strategy to Reduce its Toxicity during Embryogenesis

الموضوعات

طب

الملخص

Although carbon nanofibers (CNFs) have been implicated in biomedical applications, they are still considered as a potential hazard. Conversely, mesoporoussilica nanoparticles are generally considered as a suitable and biocompatible materialfor in-vivo use. In this study, we sought to discover a novel strategy leads as a potential approach to overcome CNFs toxicity. We fabricated conventional CNFs and novel CNFs coated with a mesoporous silica layer (MCNFs). They were synthesized bypreparing polyacrylonitrile fibers via electrospinning, followed by carbonization at 800C in an inert atmosphere. A soft templating strategy was applied to coat CNFs with amesoporous silica layer. The obtained nanofibers were then characterized by various analytical techniques. Subsequently, we used avian embryos at 3 days and its chorioallantoic membrane (CAM) at 6 days of incubation to evaluate the impact of synthesized CNFs and MCNFs on the early stage of embryogenesis and angiogenesis. We confirmed ourembryogenesis data using Drosophila melanogaster. Furthermore, we analyzed the outcome of the effect of CNFs and MCNFs on normal embryonic fibroblast cells. Ourdata show that mesoporous coating of CNFs resulted in significantly reducing both, embryotoxicity and angiogenesis of the CAM. Additionally, we elucidate key regulatorgenes of embryotoxicity induced by CNFs and MCNFs; thus, RT-PCR analysis was.

روابط وملفات

التعريف والنوع

رقم الوثيقة

09add76a-dd5f-42da-8d3d-38534efc860b

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

798372_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Ahmed Elzatahry","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Ala Eddin Al Mustafa","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Ashraf Khalil","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Ghada Ahmed Galal","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Surface Coating of Carbon Nanofiber by Mesoporous Silica: a Promising Strategy to Reduce its Toxicity during Embryogenesis

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

carbon nanofibers (CNFs) mesoporous silica layer (MCNFs)

إشراف وإعداد

الإشراف

Ahmed Elzatahry, Ala Eddin Al Mustafa, Ashraf Khalil

الإعداد

Ghada Ahmed Galal

الاقتباسات الببليوغرافية