EVALUATION OF THE OXIDATIVE STRESS IN PATIENTS WITH DIABETES MILLITUS USING SOME ENZYMATIC ACTIVITIES

الموضوعات

طب

الملخص

Diabetes mellitus is a common health problem characterized by hyperglycemia resulting from absolute or relative decrease in insulin secretion from β-cells of the islets of Langerhans in the pancreas. Diabetes mellitus is considered as a member of oxidative stress syndrome. It is associated with an imbalance between types of free radicals and scavenger systems. This study is aimed to assess the oxidative stress status in patients with type 1 or 2 diabetes mellitus disease, and to explore the good free radical marker in this state. This research was conducted in Al-Diwaniya General Hospital; Department of Chemistry, College of Science, University of Babylon; Department of Chemistry, College of Medicine, Al-Nahrain University; Iraq. This study is designed to screen the serum for certain biochemical variables. The subjects of this study were 55 patients with type 1 diabetes mellitus, 55 patients with type 2 diabetes mellitus, and 55 apparently healthy subjects were enrolled in this study. The main outcome measurements were assessment of specific biological alterations in oxidative stress syndrome: lipid peroxidation and hyperhomocysteinemia; assessment of some enzymatic antioxidants: extracellular superoxide dismutase, catalase, glutathione S-transferase, and creatine kinase; assessment of some non-enzymatic antioxidants: glutathione, vitamin E, vitamin C, and vitamin A; and assessment of minerals: iron, copper, zinc, chromium, and selenium. The results pointed out elevated levels of malondialdehyde and total homocysteine significantly in serum of patients with type 1 and 2 diabetes mellitus (DM) disease compared to control, P<0.05. Levels of lipid peroxidation showed a significant negative correlation with levels of vitamin E (r = -0.345, P<0.05), whereas there is no significant negative correlation with vitamin C and A in serum of patients with type 1 DM. While in patients with type 2 DM, the results showed a significant negative correlation with levels of vitamin C (r = -0.24, P<0.05), and a negative correlation but not significant with vitamins E and A. This study reported that extracellular superoxide dismutase and glutathione S-transferase activities are significantly higher in serum of patients with type 1 and 2 diabetes mellitus disease than that of controls, P<0.05. But there is a significant decrease in levels of catalase and creatine kinase enzyme activities in both types of diabetic patients, P<0.05. However, extracellular superoxide dismutase enzyme activity in both types of diabetic patients tends to be altered and did not show a significant correlation with levels of copper or catalase enzyme activity, while there is a significant negative correlation with level of zinc, P<0.05. This study showed that serum glutathione, vitamin E, vitamin C, and vitamin A are significantly decreased in patients with type 1 and 2 DM when they are compared with controls, P<0.05. The levels of glutathione showed different correlations, but not significant with levels of malondialdehyde, total homocysteine, and creatine kinase enzyme activity in serum of diabetic patients (with type 1 and 2 DM). Furthermore, glutathione shows a significant negative correlation with level of glutathione S-transferase activity in serum of patients with type 2 DM (r = -0.350, P<0.05), but this correlation is not significant in patients with type 1 DM. The results of this study also indicated that diabetes results in disturbance of trace elements. Thus, there is a significant decrease in levels of serum iron, zinc, chromium, and selenium in patients with type 1 and 2 DM from control, P<0.05. As well as there is a significant increase in levels of copper in serum of patients with type 1 and 2 DM from control, P<0.05. In this study, the results reported no significant difference in levels of malondialdehyde, total homocysteine, glutathione, vitamin C, copper, iron, zinc, selenium, chromium, and glutathione S-transferase, extracellular superoxide dismutase, creatine kinase, catalase activities in serum of smoker diabetic patients with type 1 and 2 DM than that of non-smoker diabetic patients, as well as levels of vitamin E in serum of smokers with type 1 DM, and levels of vitamin A in serum of smokers with type 2 DM than that of non-smoker diabetic patients. But there is a significant difference in levels of vitamin A in serum of smokers with type 1 DM, and vitamin E in serum of smokers with type 2 DM, P<0.05 than that of control. This study concluded that diabetes mellitus is a member of oxidative stress syndrome. It reduces some enzymatic and non-enzymatic antioxidants. As well as decreased levels of the antioxidants (catalase and glutathione) are good indicators to evaluate the oxidative stress syndrome in diabetic patients, and all free radical markers in this study can be used for early detection of diabetic complications. Smoking reduces the levels of antioxidants, thus increases oxidative stress syndrome, and diabetic complications in diabetic smokers are more than diabetic non-smokers.

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التعريف والنوع

رقم الوثيقة

098ce336-52fa-485a-90d3-75f92fa3218b

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1002076_1.pdf

بيانات النشر

ترجمة العنوان

تقيم الحالة التاكسدية لمرضى أداء السكري باستخدام بعض المؤشرات الانزيمية

ألقاب المؤلفين

[{"name_ar":"Mufeed J.Ewadh","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Oda M. Yasser ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"فردوس عباس جابر","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

EVALUATION OF THE OXIDATIVE STRESS IN PATIENTS WITH DIABETES MILLITUS USING SOME ENZYMATIC ACTIVITIES

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

يعتبر داء السكري من المشاكل الصحية الشائعة، والذي يتميز بارتفاع مستوى السكر الناجم عن الفقدان أو النقص النسبي للأنسولين الذي تفرزه خلايا بيتا في جزر لانكرهانس في البنكرياس.يعد مرض السكري من العناصر المتلازمة Syndrome لضغط المؤكسدات ذات الصلة بالتوازن بين أنواع الأوكسجين الفعال ونظام الكاسحات Scavengers.تهدف هذه الدراسة إلى إظهار مستوى ضغط المؤكسدات للمصابين بالنوع الأول والنوع الثاني من مرض السكري وكشف أقوى مؤشر للجذور الحرة في حالة هذا المرض.تم إجراء هذه الدراسة في مستشفى الديوانية العام وفي قسم الكيمياء / كلية العلوم / جامعة بابل وفي قسم الكيمياء / كلية الطب / جامعة النهرين, العراق. صممت هذه الدراسة لغرض الكشف عن بعض المتغيرات الكيميائية الحياتية لمصل الدم. خضع 50 مريضا بالسكري من النوع الأول، و50 مريضا بالسكري من النوع الثاني، و50 شخصا سويا لفحوصات هذه الدراسة.القياسات الرئيسية في هذه الدراسة هي قياس التغيرات البايولوجية النوعية لمتلازمات الحالة التأكسدية (أكسدة الدهون وارتفاع مستوى الهوموسستين) وقياس فعالية بعض مضادات الأكسدة الإنزيمية Enzymatic Antioxidants: إنزيم Superoxide Dismutase وإنزيم Catalase وإنزيم Glutathione S-transferase و إنزيم Creatine Kinase وقياس بعض مضادات الأكسدة غير الإنزيمية Non-enzymatic Antioxidants: الكلوتاثايون Glutathione وفيتامين Eوفيتامين C وفيتامين A وقياس العناصر التالية: الحديد والنحاس والزنك والكروم والسيلينيوم.سجلت نتائج هذه الدراسة ظهور ارتفاع ملحوظ في مستوى المالون داي ألدهايد (الناتج النهائي لأكسدة الدهون) والهوموسستين الكلي في مصول دم المرضى المصابين بالسكري من النوع الأول والثاني عن ذلك المستوى الذي ظهر في المجموعة الضابطة. أظهرت مستويات أكسدة الدهون ارتباط معنوي سالب جدير بالملاحظة مع مستويات فيتامين E (r = - 0.345, P

كلمات الباحثين

Diabetes mellitus, oxidative stress, antioxidants, free radicals, enzymes, vitamins, trace elements

إشراف وإعداد

الإشراف

Mufeed J.Ewadh, Oda M. Yasser

الإعداد

فردوس عباس جابر

الاقتباسات الببليوغرافية