The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines

الموضوعات

علوم بحتة وطبيعية

الملخص

Acute Myeloid Leukemia (AML) is a type of cancer characterized by the formation of faulty myelogenous cells with morphological heterogeneity and cytogenic aberrations leading to a total loss of their function. In the midst of the struggle to find an effective and safe AML treatment, vitamin E derivatives, mainly tocopherols were previously considered as potential anti-tumor compounds. Recently, other isoforms of vitamin E, namely tocotrienols have been proposed as potential potent anti-cancerous agents, displaying promising therapeutic effects in different cancer types. The aim of the present study is to investigate the anti-cancerous effect of γ-tocotrienol, through examining the extent of its effects on the growth of human-derived AML cell lines in vitro, and deciphering the underlying molecular mechanisms. This was achieved by performing MTS cell viability assays, propidium iodide (PI) based cell cycle analysis, flow cytometry-based annexin V/PI apoptosis staining assay, cell death detection ELISA, western immunoblotting, and reactive oxygen species detection assay. Analysis of our results show that γ-tocotrienol exhibits time and dose-dependent anti-proliferative, pro-apoptotic and antioxidant effects on U937 and KG-1 cell lines, through the upregulation of several proteins involved in the intrinsic apoptotic pathway.

روابط وملفات

الوثيقة في المستودع الخارجي عرض في AskZad

التعريف والنوع

رقم الوثيقة: 091056cb-b6f0-434a-a21c-3f47dab4379e
رقم العقد: 0
نوع الوسائط: Crawler
نوع المحتوى: الرسائل العلمية
صيغة المصدر: رسائل ماجيستير
نوع الملف: pdf text
أسماء الملفات: 2212881_1.pdf

بيانات النشر

ألقاب المؤلفين: [{"name_ar":"Maya Riad Mohamad","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Sandra Rizk ","title_ar":"اشراف","title_en":"Supervision"}]
اللغة: English

المصدر والدورية

اسم المصدر: The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines

المحتوى والصفحات

عدد الصفحات: 0
كلمات الباحثين: derivative gamma

إشراف وإعداد

الإشراف: Sandra Rizk
الإعداد: Maya Riad Mohamad

الاقتباسات الببليوغرافية

APA

Maya Riad Mohamad و Sandra Rizk . (2019). The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. أطروحة(رسائل ماجيستير). مدرسة الآداب والعلوم-الجامعة اللبنانية الأميركية. لبنان.

MLA

Maya Riad Mohamad و Sandra Rizk . The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. 2019. مدرسة الآداب والعلوم-الجامعة اللبنانية الأميركية، رسائل ماجيستير.