Nephroprotective Effect of Quercetin from Cisplatin Induced Acute Nephrotoxicity in Male Rats

الموضوعات

طب

الملخص

The nephroprotective effect of quercetin (100 and 200 mg/kg orally) was investigated by using 10.5 mg/kg b.w cisplatin, I.P to induce nephrotoxicity in rats. Thirty male rats were divided randomly into five groups (6 in each group). The first group received 5 ml of normal saline I.P as a single dose and considered as a control group. The second group was received 10.5 mg /kg cisplatin I.P as a single dose and considered as an active control group. The third ( protection) group given 100 mg /kg b.w quercetin orally 8 hr before and 24 hr after cisplatin injection. The fourth (protection) group given 200 mg /kg b.w quercetin orally 8 hr before and 24 hr after cisplatin injection. While the fifth group received 200 mg/kg b.w quercetin orally8 hr before and 24 hr after normal saline injection. Blood samples were collected and used to determine blood urea and serum creatinine. One of the kidneys used to determine the tissue oxidative parameter (GSH), lipid peroxidation marker (MDA) and inflammatory marker (TNF- α). While the second kidney was fixed and used to determine the histopathological changes. Blood urea and serum creatinine concentrations were significantly elevated (P<0.05) in the cisplatin only treated group as compared to the normal group, while the concentrations of blood urea and serum creatinine in the quercetin received groups significantly decreased (P<0.05) as compared with the active control group. Moreover, cisplatin induced decline of renal tissue antioxidant enzyme (GSH) activity, while quercetin was significantly protected the level of GSH (P<0.05). Cisplatin significantly increase MDA and TNF-α (P<0.05) concentration referred to increased lipid peroxidation and inflammation respectively. While in quercetin protection group MDA and TNF-a significantly decrease (P<0.05) as compared to cisplatin group. The histopathological examination explained the nephrotoxic effect of cisplatin. The proximal tubules showed coagulative necrosis in the cortico-medulary junction and the tubular epithelial cells casts in the lumen of the proximal tubules , these events significantly did not exist in normal and quercetin protection groups.

روابط وملفات

التعريف والنوع

رقم الوثيقة

08a316cf-565f-489d-ae44-9a0465384d47

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1000218_1.pdf

بيانات النشر

ترجمة العنوان

التأثير الوقائي لمادة الكويرسيتين من الأثر السمي الحاد لعقار السيسبلاتين على الكلية في ذكور الجرذان

ألقاب المؤلفين

[{"name_ar":"بتول أمين الخفاجي","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"سالم فايز كاظم","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"نسرين جلال محمد","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Nephroprotective Effect of Quercetin from Cisplatin Induced Acute Nephrotoxicity in Male Rats

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Nephroprotection, Quercetin, Cisplatin, Kidney Function, Oxidative Stress, MDA, GSH, TNF-α, Histopathological Changes

إشراف وإعداد

الإشراف

بتول أمين الخفاجي, نسرين جلال محمد

الإعداد

سالم فايز كاظم

الاقتباسات الببليوغرافية