Antimicrobial activities and phytochemical analysis of Verbascum fruticulosum

الموضوعات

علوم بحتة وطبيعية

الملخص

Background:Phenolic compounds are a group of secondary metabolites which are found in many medicinal plants and have a critical role in defense against pathogens and inhibiting different oxidative stress related diseases like cancer.This study screened Verbascum fruticulosum phytoconstituents and evaluated the total phenol, tannin, and flavonoid content. In addition, it evaluated the antioxidant and antimicrobial activities of these compounds.Methodology: Antioxidant activity was analysed using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method, while phyto-constituents, total phenols, tannins, and flavonoids were screened and evaluated using standard analytical methods for phytochemicals. Antimicrobrial activity was evaluated using a broth micro-dilution method against American Type Culture Collection (ATCC) reference strains including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecium, Shigella sonnei, Candida albicans, and a clinical isolate of multidrug resistant Staphylococcus aureus (MRSA)., except Epidermophyton floccosum strain where was used agar dilution method.Results: The phytochemical screening showed that all extracts except n-hexane contained phenol and tannins. Only aqueous and acetone extracts contained flavonoids, so they had the highest antioxidant activity. Total phenol and flavonoid content was highest in acetone extracts, while methanolic extract showed the highest total tannin content. Antibacterial tests showed that the best inhibition activity of the methanol and acetone extracts were against P. arginosa with minimum inhibitory concentrations (MICs) of 1.56 mg/ml in both cases. Furthermore, the acetone extract also showed the highest inhibiting activity against S. aureus and E. coli with MICs of 6.25 mg/ml. Aqueous extract showed the highest inhibition activity against S. aureus (MRSA) with a MIC value of 3.125 mg/ml. The highest antibacterial activity of the methanol andn-hexane extracts were against E. faecium and S. sonnei with MICs of 3.125 mg/ml, in each respective assay. For antifungal inhibition the best activity was shown by the n-hexane extract against E. flacosum with a MIC value of 1.56 mg/ml.Conclusion: Aqueous extracts showed the highest level of antioxidant activity, methanol and acetone extracts showed the highest antibacterial activity. While, n-hexane was considered the best antifungal extract. High level of phenolic compounds found in Verbascum fruticulosum that could be included in new antibiotic and anti-cancer drugs designs.

روابط وملفات

التعريف والنوع

رقم الوثيقة

08811f9c-f5c6-4311-b801-7cc69244ccbb

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1611954_1.pdf

بيانات النشر

ترجمة العنوان

دراسة النشاط المضاد للميكروبات والتحليل الكيميائي النباتي لنبات البوصير الشجيري

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Antimicrobial activities and phytochemical analysis of Verbascum fruticulosum

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

رائد الكوني, نضال جرادات

الإعداد

صالح سليم فهمي فارس

الاقتباسات الببليوغرافية