Correlation between membrane fluidity cellular development and stem cell differentiation

الموضوعات

علوم بحتة وطبيعية

الملخص

Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.

روابط وملفات

التعريف والنوع

رقم الوثيقة

084b4a05-5d30-484d-8e72-13e409581d31

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1699629_1.pdf

بيانات النشر

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Correlation between membrane fluidity cellular development and stem cell differentiation

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

development , Differentiation, Neuron , stem cell

إشراف وإعداد

الإشراف

الصحراوي شايب

الإعداد

باكيزا كمال نوتسي

الاقتباسات الببليوغرافية