دراسة مقارنة لمعايرة الكوليسترول منخفض الكثافة بالطريقة الانزيمية على الجهاز الالي والطريقة الحسابية

الموضوعات

طب

الملخص

روابط وملفات

التعريف والنوع

رقم الوثيقة

07e2216e-1ce6-40ce-a68c-17a1c77a5ce6

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1961665_1.pdf

بيانات النشر

ترجمة العنوان

A comparative Study of low-density cholesterol determination by enzymatic method using automatic device and calculative method

ألقاب المؤلفين

[{"name_ar":"فتون لبابيدي","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"محمد عماد بليد","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

دراسة مقارنة لمعايرة الكوليسترول منخفض الكثافة بالطريقة الانزيمية على الجهاز الالي والطريقة الحسابية

المحتوى والصفحات

عدد الصفحات

84

ترجمة الملخص

Low density (LDL) is considered the more coronary heart diseases (CHD).So, its routine measurement is recommended in the evaluation and management of .There are several methods to measure LDL-cholesterol, but the enzymatic method (direct) calculation method ( equation) are the most practical one in the clinical laboratory.This study aimed to compare between the enzymatic and calculation method in term of precision and reliability of the results.Patients and methods:The present study was carried out on 300 patients, patients have been chosen randomly from Aleppo university hospital departments.The samples were classified into three groups:Group 1: patients with TG < 200 mg/dl or < 2.26 mmol/l.Group 2: patients with TG between 200 –400 mg/dl or 2.26 –4.52 mmol/l.Group 3: patients with TG > 400 mg/dl or<4.52 mmol/l.LDL-cholesterol was measured by the enzymatic (direct) method. Tc, HDL-c, and TG levels were measured .The LDL-c was estimated by Formula as -c (mg/dl) = (Tc) –(HDL-c) –(TG/5)Results:The LDL concentrations measured by enzymatic method were compared to those calculated by ( Formula).The results were as follows:We found that, the precision of the results was good in both methods, but it was better in the enzymatic method as (CV) was between (0.75 –1.04)%, while (CV) was (1.12 –2.28)% in the calculation method. 79 The correlation was good between the results of the enzymatic method and calculation method for samples with TG level less than 400 mg/dl and (r) was between (0.961 –0.911) (for group 1,2).But the correlation between the results of two methods wasn't samples with TG levels more than 400 mg/dl and r = 0.763 (for group 3).There significant difference between the results of the enzymatic method and that of the calculation method with TG level more than 400 mg/dl (p <0.05), but no significant difference was observed for the samples with TG level less than 400 mg/dl (p>0.05).Conclusion:The enzymatic method is better than the calculation method for the measurement of LDL-c because: It's easy, rapid and economic method. It gives reliable results with , while the calculation method at triglyceride level more than 400 mg/dl, or in the patients with (Type III) L-c measured directly and not estimated from other parameters.The convenience of measuring LDL-c in non fasting blood samples.

إشراف وإعداد

الإشراف

محمد عماد بليد

الإعداد

فتون لبابيدي

الاقتباسات الببليوغرافية