The prevalence of cytomegalovirus antibodies in kidney candidate recipients, kidney candidate donors and blood donors at Ahmed Gasim Renal Transplant Centre

الموضوعات

طب

الملخص

The prevalence of CMV antibody of IgG and IgM isotypes amongpretrasplant kidney recipient (group 1), kidney candidate donors (group 2)and healthy blood donors (group 3) was studied at Ahmed Gasim cardiacsurgery and Renal Transplant Centre inKhartoum North, between Januaryand December 2005. Blood samples were collected from the various groupsand sera, were screened by indirectELISA for group 1, 2 and 3 andImmuno chromatography test (ICT) for group 4, 5 and 6 for the presence ofCMV antibodies using the appropriate conjugate of antihuman (Fc) portionof the respective immunoglobulin class.Pretransplant screening of the kidney patients waiting renal transplant(group 1) revealed that 96% of the patients have IgG CMV antibodies and10% have IgM antibodies. Significant high titer of IgG (OD> 2.0) probablyindicates repeated infection or reactivation of latent infection, where as IgMantibodies indicate recurrent primary infection. In the healthy kidneycandidate donors (Group 2)92% showed the presence of the IgG antibodyto CMV and 17 % revealed the presence of IgM. On the other hand thepresence of CMV antibodiesin healthy blood donors (group 3) was 84% for1gG and 22% for 1gM. We also observed that the presence of 1gMantibodies varied in the various age groups. It was high in children andyoung adult and was almost absent in elderly suggesting that seroconvertionis an ongoing process.The prevalence of CMV IgG antibodies were also determined usingICT, in kidney candidate recipients (group 4), healthy kidney candidatexidonors (group 5), healthy blood donor (group 6), were found 92.5%, 87.5%and 82.5%, respectively. The correlation between ICT and ELISA for thedetection of CMV IgG antibodies was 96%. Therefore, both assay weresensitive and specific for the detection of CMV IgG antibodies.From this study it appeared thatthe high prevalence of CMVinfection in the various groups studied might contribute to organ transplantrejection.

روابط وملفات

التعريف والنوع

رقم الوثيقة

07d36304-6f0d-4c09-98e2-701a7d9a05e6

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1638496_1.pdf

بيانات النشر

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

The prevalence of cytomegalovirus antibodies in kidney candidate recipients, kidney candidate donors and blood donors at Ahmed Gasim Renal Transplant Centre

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

عوض الكريم

الإعداد

اسامة بوشيرة

الاقتباسات الببليوغرافية