Interactions between Cryptosporidium parvum and the Intestinal Ecosystem

الموضوعات

علوم بحتة وطبيعية

الملخص

Cryptosporidium parvum is an apicomplexan protozoan parasite commonly causingdiarrhea, particularly in infants in developing countries. The research challenges faced inthe development of therapies against Cryptosporidium slow down the process of drugdiscovery. However, advancement of knowledge towards the interactions of the intestinalecosystem and the parasite could provide alternative approaches to tackle the disease.Under this perspective, the primary focus of this work was to study interactions betweenCryptosporidium parvum and the intestinal ecosystem in a mouse model. Mice were treatedwith antibiotics with different activity spectra and the resulted perturbation of the nativegut microbiota was identified by microbiome studies. In particular, 16S ampliconsequencing and Whole Genome Sequencing (WGS) were used to determine the bacterialcomposition and the genetic repertoire of the fecal microbial communities in the mousegut. Following alteration of the microbial communities of mice by application of antibiotictreatment, Cryptosporidium parasites were propagated in mice with perturbed microbiotaand the severity of the infection was quantified. This approach enabled the prediction ofthe functional capacity of the microbial communities in the mouse gut and led to theidentification of bacterial taxa that positively or negatively correlate in abundance withCryptosporidium proliferation.

روابط وملفات

التعريف والنوع

رقم الوثيقة

06ee326f-2228-4050-b03b-af92e7f0d9cf

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1699543_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Arnab Pain","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Olga Douvropoulou","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Interactions between Cryptosporidium parvum and the Intestinal Ecosystem

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Cryptosporidium, dysbiosis , microbiota

إشراف وإعداد

الإشراف

Arnab Pain

الإعداد

Olga Douvropoulou

الاقتباسات الببليوغرافية