Genome-wide analysis of multidrug resistant shigella SPP. Isolated from clinical setting in Lebanon

الموضوعات

علوم بحتة وطبيعية

الملخص

Shigella spp. are Gram-negative rod-shaped bacteria belonging to the familyEnterobacteriaceae and are known to be a major cause of bacillary dysenteryworldwide. In this study, whole-genome sequencing was used for the molecularcharacterization of ESBL producing Shigella spp. isolates collected from hospitals inLebanon. PCRs were performed to detect β-lactam resistance gene reservoirs and toidentify genes involved in virulence and host adaptation. PCR-based replicon typing(PBRT) was performed to identify patterns of plasmid distribution. For comparativeanalysis, multi-locus sequence typing (MLST), whole-genome based SNP analysis,Pan-genome analysis and pulsed-field gel electrophoresis (PFGE) were performed todetermine the phylogenic relatedness of the isolates and to trace evolutionary lineages.Our study revealed that, eight of the isolates were ESBL producers; resistance to β-lactam was mainly manifested by the presence of blaCTX-M-15, blaCTX-M-3, and blaTEM-1Bgenes. Inc I1α and IncFII were the prevalent incompatibility groups, with IncI1α beingdetected in all the isolates, and IncFII in eight of the isolates. β-lactam resistancedeterminants were found mainly on these two plasmids. class 2 integron was the mostcommon integron detected (nine of the isolates) whereas class 1 was only seen in twoof the isolates. The ten sequenced isolates were distributed on three multi-locussequence types (MLST) (ST152, ST145 and ST245) with ST152 being the predominant. Our study focuses on important virulence determinants and effectorproteins used by the bacterium to destroy the host. Individual PCRs were performedto detect the presence of virA (motility), ial (invasion), ipaH (immune systemmodulation), set and sen (enterotoxins). The study provided an overview of the majorplasmid families circulating in multidrug resistant Shigella in Lebanon. The resultsobtained revealed the role of integrons and gene cassettes in the dissemination ofantimicrobial resistant determinants in Shigella and gave an insight of the phylogenicrelationships between the undertaken isolates and other worldwide-recovered representatives. This study and through the use of genome-based approaches helpedin better understanding the diversity of Shigella, its pathogenesis and drug resistanceat the molecular level, and the risk factors associated with the mobilization of plasmidsbetween Shigella spp. and other enteric pathogens

روابط وملفات

التعريف والنوع

رقم الوثيقة

062afae9-3522-408c-918b-41b9e1e43985

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2212874_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Sima Tokajian","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Yara J. Salem","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Genome-wide analysis of multidrug resistant shigella SPP. Isolated from clinical setting in Lebanon

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Isolated from clinical

إشراف وإعداد

الإشراف

Sima Tokajian

الإعداد

Yara J. Salem

الاقتباسات الببليوغرافية