Polyphenol Profiling And Bioactivities Of Date Seed: A Comparative Assessment Of Extraction Techniques In Different Date Seed Varieties

الموضوعات

علوم تطبيقية وتكنولوجية

الملخص

Plant-waste-derived bioactive compounds have generated growing interest recently. In this context, this project examined the phenolic content, antioxidant capacity, antimicrobial activity, and antibiofilm capability of the seed extract of Phoenix dactylifera . Six date seed varieties (Fardh, Sukarri, Negal, Majdool, Zahidi, and Khadrawy) were studied. In this study, a novel ultrasound-assisted extraction technique is compared with the conventional extraction method (maceration using 80% ethanol), taking into account both the quality and quantity of polyphenols. Among the primary objectives, the ultrasound-assisted extraction (UAE) of polyphenols was optimized using response surface methodology (RSM), aiming for maximum TPC and DPPH radical scavenging. By studying three extraction parameters, ethanol concentration (0-80%), time (1-15 min), and amplitude (10-80%), the optimum extraction conditions of 80% ethanol, for 6 min, at amplitude 80%, were obtained. Experimental validation of the optimum conditions predicted by the designed model showed R 2 > 96% for both responses. The comparison study between date seed varieties proved that the UAE method was the most efficient technique for producing higher phenolic extracts (TPC ranged from 50.44±0.65 to 381.90±13.49 mg GAE/100g DSP) with powerful antioxidant activity compared to the conventional method (47.74±0.31 to 287.23±3.97 mg GAE/100 g DSP). Findings of the antibacterial activity against pathogens supported their significant capabilities to inhibit pathogens with low MIC values. The most promising results were found with the ultrasonic extract of Sukkari against Enterococcus faecalis (ATCC 19433) with MIC 1.03 mg/mL, and ultrasound extracts of Negal against Staphylococcus aureus (ATCC 6538) with MIC 1.87 mg/ml. In addition, results indicated the potential of these DSEs to inhibit biofilm formation with difficulty in eliminating the existing biofilms. The characterization of the polyphenols using UHPLC and LC–ESI-MS/MS revealed 14 compounds (gallic acid, (+)-catechin, 1,2- dihydroxybenzene, chlorogenic acid, 4-hydroxybenzoic acid, caffeic acid, vanillic acid, flavone, t-ferulic acid, rutin hydrate, t-cinnamic acid, p -coumaric acid, syringic acid, and sinapic acid). Furthermore, the FTIR analysis confirmed the detection of potentially active components in all DSEs. This research could significantly contribute to the development of functional foods and nutraceuticals. The findings of this study may lead to the creation of novel dietary supplements and pharmaceuticals that provide enhanced health benefits by optimizing extraction methods and thoroughly characterizing the bioactive compounds found in date seeds.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0602e4f7-59e5-425c-b4d6-cae2200a7052

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

840795_1.pdf

بيانات النشر

ترجمة العنوان

تحليل الفينولات والنشاطات البيولوجية لبذور التمر: دراسة مقارنة لتقنيات الاستخلاص في أصناف مختلفة من نوى التمر

ألقاب المؤلفين

[{"name_ar":"Fatima Ibrahim Albedwawi","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Raman Bhaskaracharya","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Polyphenol Profiling And Bioactivities Of Date Seed: A Comparative Assessment Of Extraction Techniques In Different Date Seed Varieties

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Raman Bhaskaracharya

الإعداد

Fatima Ibrahim Albedwawi

الاقتباسات الببليوغرافية