Analysis of Serine Proteases involved in Drosophila melanogaster immunity and identification of potential biocontrol agents

الموضوعات

علوم بحتة وطبيعية

الملخص

Although the fruit fly Drosophila melanogaster is not an agricultural pest and does not transmit pathogens to humans, it has been used as a model to decipher innate immune responses and to study host-pathogens interactions. Antimicrobial peptides are a major component of insects’ immunity and their production is mainly controlled by two pathways: Toll and IMD. In response to fungal or Gram-positive bacterial infections, the Toll signaling pathway is activated by a cascade of Serine Proteases (SPs) leading to the cleavage of the cytokine Spz, the Toll ligand. Several SPs have been shown to act upstream of Toll, linking microbial recognition to the cleavage of Spz. Other SPs have also been shown to mediate the flies’ melanization reaction. Serine Protease inhibitors or serpins (SPN) are negative regulators of melanization and AMPs production. Large numbers of SPs and several serpins remain unexplored and the complexity of proteolytic cascades operating upstream of Toll during the flies’ immune response is not completely understood. Using an in vivo RNAi approach, we screened the unexplored SPs for their potential role in Toll activation. We also collected different naturally occurring fungi from dead arthropods and tested their pathogenicity on lab insect models in the aim of a potential utilization as new biological pest control agents.

روابط وملفات

التعريف والنوع

رقم الوثيقة

053e7e48-df35-44f2-a7e9-87d5570db894

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

2026234_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Sana Allam Jaber","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Zakaria Kambris","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Analysis of Serine Proteases involved in Drosophila melanogaster immunity and identification of potential biocontrol agents

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Show simple item record dc.contributor.advisor Kambris, Zakaria dc.contributor.author Jaber, Sana dc.date.accessioned 2020-09-23T11:17:19Z dc.date.available 2020-09-23T11:17:19Z dc.date.issued 2020-09-23 dc.identifier.uri http://hdl.handle.net/10938/21979 dc.description Osta Mike, Knio Khouzama, Osman Dani, Hijazi Assia dc.description.abstract Although the fruit fly Drosophila melanogaster is not an agricultural pest and does not transmit pathogens to humans, it has been used as a model to decipher innate immune responses and to study host-pathogens interactions. Antimicrobial peptides are a major component of insects’ immunity and their production is mainly controlled by two pathways: Toll and IMD. In response to fungal or Gram-positive bacterial infections, the Toll signaling pathway is activated by a cascade of Serine Proteases (SPs) leading to the cleavage of the cytokine Spz, the Toll ligand. Several SPs have been shown to act upstream of Toll, linking microbial recognition to the cleavage of Spz. Other SPs have also been shown to mediate the flies’ melanization reaction. Serine Protease inhibitors or serpins (SPN) are negative regulators of melanization and AMPs production. Large numbers of SPs and several serpins remain unexplored and the complexity of proteolytic cascades operating upstream of Toll during the flies’ immune response is not completely understood. Using an in vivo RNAi approach, we screened the unexplored SPs for their potential role in Toll activation. We also collected different naturally occurring fungi from dead arthropods and tested their pathogenicity on lab insect models in the aim of a potential utilization as new biological pest control agents. dc.language.iso en_US dc.subject Drosophila melanogaster, Serine proteases, Toll pathway, Disease-vectors, Fungi, biocontrol agent

إشراف وإعداد

الإشراف

Zakaria Kambris

الإعداد

Sana Allam Jaber

الاقتباسات الببليوغرافية