In vitro and In vivo Activity of Some Antibacterial Agents ;Alone and in Combination Against Multi Drugs Resistant Pseudomonas aeruginosa Isolated from Burns Infections in Babylon

الموضوعات

طب

الملخص

This study included 70 swabs collected from burned patients of both sexes, males and females of different ages attending the burn unit at Al-Hilla Teaching Hospital in Al-Hilla city during a period of three months from October to December 2011. The results revealed that 100% of samples showed positive results for bacterial culture. The results indicated that 26/70 (37.01%) of samples were Pseudomonas aeruginosa and 44/70 (62.9%) revealed other types of bacteria, which included 23/70 (32.9%) Staphylococcus spp. and 21/70 (30%) Escherichia coli. The susceptibility of Pseudomonas aeruginosa towards six antibiotics included in this study by Disk Diffusion Test showed that all P. aeruginosa isolates (100%) exhibited resistance to Rifampin, Gentamycin, Pipracillin, and Tobramycin, while they were sensitive to Meropenem and Amikacin. In addition, P. aeruginosa revealed remarkable susceptibility toward natural products, Garlic Vinegar, but it was not affected by Garlic Oil. The minimum inhibitory concentration (MIC) for the six antibiotics using the Pouring method was carried out. The results showed that P. aeruginosa isolated from patients was resistant to most of the antibiotics used in this study. MIC values were 64 μg/ml for Amikacin and Meropenem, 256 μg/ml for Rifampin, while no inhibition zone was observed on the bacterial culture tested with Gentamycin, Pipracillin, and Tobramycin. To detect the response of Multi-Drug Resistant isolates of P. aeruginosa towards a combination of antibiotics with each other using the Well Diffusion method, it was shown that Meropenem and Amikacin revealed high potent activity against the tested organism when combined with other antibiotics, indicating synergy and additive action. However, Amikacin and Tobramycin revealed an inhibition zone of 43 mm in diameter. Meropenem combined with Pipracillin and Amikacin showed an inhibition zone of 43 mm and 37 mm in diameter, respectively. According to these results, the six antibiotics were employed for in vivo experiments, since they were used for treatment of experimentally infected animals alone and in combination. The response of experimentally infected animals in recovery from experimentally burned infection was remarkable when combined antibiotics were used for treatment in comparison to the use of single antibiotics, which indicated that a synergy reaction was observed, and the results were in accordance with the Well Diffusion method.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0468f4f4-b2eb-4a2b-967e-046645e74ecc

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1000480_1.pdf

بيانات النشر

ترجمة العنوان

فعالية مضادات حيوية مختارة منفردة ومركبة خارج وداخل الجسم الحي ضد بكتريا Pseudomonas aeruginosa متعددة المقاومة للمضادات والمعزولة من أخماج حروق في بابل

ألقاب المؤلفين

[{"name_ar":"حبيب صاحب نهر","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"زهراء قيس سلمان السالم","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

In vitro and In vivo Activity of Some Antibacterial Agents ;Alone and in Combination Against Multi Drugs Resistant Pseudomonas aeruginosa Isolated from Burns Infections in Babylon

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Burns, Pseudomonas aeruginosa, Staphylococcus spp, Escherichia coli, Multi-Drug Resistance, Disk Diffusion Test, Minimum Inhibitory Concentration (MIC), Antibiotic Synergy, Natural Products, Infection Treatment

إشراف وإعداد

الإشراف

حبيب صاحب نهر

الإعداد

زهراء قيس سلمان السالم

الاقتباسات الببليوغرافية