دراسة مناعية, كيموحيوية وجُزيئية للمُخمجين و المُلقحين بفيروس التهاب الكبد نمط - ب- في محافظة بابل

الموضوعات

طب

روابط وملفات

التعريف والنوع

رقم الوثيقة

045c4b9d-dc37-4c2a-991c-667457c521e5

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1000346_1.pdf

بيانات النشر

ترجمة العنوان

Immunological, Biochemical and Molecular Study of Hepatitis B virus Infected and Vaccinated peoples in Babylon province

ألقاب المؤلفين

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اللغة

Arabic

المصدر والدورية

اسم المصدر

دراسة مناعية, كيموحيوية وجُزيئية للمُخمجين و المُلقحين بفيروس التهاب الكبد نمط - ب- في محافظة بابل

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

Hepatitis B virus (HBV) infection is a major public health problem and its outcome depends on the kinetics of the virus host interaction and in particular on the strength of the immune response. This study was done to illustrate some immunological, biochemical and molecular markers of infected hepatitis B virus population and effectiveness of HBV vaccine in vaccinees groups . This study was conducted from September , 2011 to October, 2012 .ELISA assay was used for detection of hepatitis B virus surface antigen as well as IgM- anti hepatitis B-core antigen and anti-HBeAg were used in this study for detection of HBV and its immunological response in infected population . The result revealed that only 48 out of 1200 subjects (11-60) years old had HBsAg positive which represent (4%) with high frequency in male( 2.1%/), than female(1.3%) group. the test of IgM-anti HBcAg revealed the highest percentage of acute infection was detected within age group (31–40) years old which reach to 77.7% whereas, anti-HBeAg revealed the highest percentage of chronic infection within age group (51–60) years old which reach to 15.4%.Biochemical tests of liver function revealed enhancement of Alanine aminotransferase which reach the highest level (608.8IU/L)within age group (51–60) years old in male and 380.3IU/L within age group (31–40) years old in female . The highest level of aspartate aminotransferase was 532.2 IU/L within age group (51–60) years old in male, whereas in female it was reached to 367.2 IU/L within age group (41–50) years old . The study also reflected enhancement o f IL-2 level in all infected groups in comparison with normal healthy control group (P<0.05) . The highest level for it was reached to 33.84pg/ml and 41.25pg/ml in male and female respectively. The IL-4 level also revealed a significant enhancement of its level in all infected groups in comparison with normal healthy control group (P<0.05). The highest level for it was reached to 20.40pg/ml and 21.44pg/ml in male and female respectively . The study also revealed enhancement of INF-α level in all infected groups in comparison with normal healthy control group (P<0.05). It reach its higher level which was 25.55pg/ml and 23.22pg/ml male and female respectively. This study was also include measurement of anti- HBsAg level to detect the immune response in vaccinees . It was found that significant differences in the antibodies levels according to post vaccination periods and the highest antibodies level in male and female was reached after 90 days post 3rd dose of vaccination , it was 124.85 mIU/ml and 155.94 mIU/ml in male and female respectively. There was also significant differences in (IL-2, IL-4 and INF-α ) cytokines it was found that the highest level of IL-2 was reached 27.21 pg/ml and 33.94 pg/ml 90 days post 3rd dose of vaccination in male and female respectively. Also IL-4 reached reached its higher level which was 12.95pg/ml and 13.23pg/ml in male and female respectively 90 days post 3rd dose of vaccination. On the other hand the highest level of IFN-α was reached 12.60pg/ml in male which was detected 60 days post 3rd dose of vaccination. Whereas it highest level in female was reached to 15.60pg/ml was detected 90 days post 3rd dose of vaccination . The study was also included molecular diagnosis for viral load in plasma of infected subjects , the highest viral load was 21.1×105 copies/ml , the lost viral load was 2.7×105copies/m

كلمات الباحثين

فيروس التهاب الكبد ب، العلامات المناعية، العلامات الكيميائية الحيوية، الحمض النووي الفيروسي، لقاح التهاب الكبد ب، الحمل الفيروسي

إشراف وإعداد

الإشراف

عبد النبي جويد المعموري, يونس عبد الرضا الخفاجي

الإعداد

ايام محمد صالح علي العامود

الاقتباسات الببليوغرافية