Bacteriological and immunological study of the prostatitis patients in Thi-Qar province

الموضوعات

طب

الملخص

In the present study, the urine and blood specimens were collected from 60 patients suffering from prostatitis and from (20) apparently healthy man as control in Al-Hussein Teaching Hospital in Thi-Qar province , Among the patients the prostatitis was occurred mainly at the age group ( 15-45 ) years in 34 (56.67 % ) of patients with significant differences ( p< 0.001 ) followed by the age group (>45-65) years in 17 (28.33 %) patients and the age group ( > 65 ) years in the 9 patients ( 15 % ) . The bacterial infections was detected in urine specimens by culture and PCR techniques after the prostatic massage. In the culture , the bacterial growth was detected in 11 (18.33%) of specimens including (10) isolates belong to Escherichia coli and (1) isolate of Enterobacter aerogenes . While , PCR technique by using universal 16S rRNA primer ( B27f-U1492r ) shown a positive bacterial signals in 23 (38.33%) of specimens with the high specificity and sensitivity than culture method ( p<0.001) . The incidence of Chlamydia trachomatis , Ureaplasma urealyticum and Mycoplasma genitalium was identified by using orf8 , ureA-B and mgpa gene primers respectively . M. genitalium was detected in 4 cases which represents (17.391 %) of cases followed by C. trachomatis in 3 cases which represents ( 13.043 %) and U. urealyticum was identified in 1 case (4.437 % ) of total specimens with high significant differences (p<0.001). While four (17.391%) PCR positive bacterial signals that detected by universal primer B27-U1492 were undiagnosed by all specific primers that used in the current study . According to the results of culture method and PCR techniques , the incidence of bacterial species in the clinical specimens of bacterial prostatitis revealed that E. coli was a common bacterial cause in 10 ( 43.47% ) cases with a high significant differences (P<0.001) , Followed by M. genitalium and C. trachomatis which diagnosed in 4 (17.39%) and 3 (13.04%) , respectively . The Enterobacter aerugenes and U. urealiticum were recorded in 1 ( 4.34% ) for each one with no significant differences among them . While four PCR positive cases by B27-U1492 16S rRNA only assigned as unknown bacteria . So, depending on the results above , bacterial prostatitis was diagnosed in 23 (38.33%) patients . While , nonbacterial prostatitis (CP/CPPS) was diagnosed in 37 (61.67%) of the patients . The isolated bacteria showed high sensitivity to amikacin where 9 ( 81.81% ) of isolates were sensitive to this antibiotic with high significant differences . While , the isolates shown moderate sensitivity to gentamicin and ciprofloxacin 4 and 3 ( 36.37 and 27.28 )% of isolates respectively and low antibiotic sensitivity was shown against cefotaxime and azithromycin (18.19%) for each one . All isolated bacteria exhibit completely resistance to ampicillin and co-trimoxazole , two isolates were shown resistance to all tested antibiotics . MIC were determined in the present study against susceptible isolates , CN has the lower MIC value ( 1.82 )μg/ml followed by AK (2.64) μg/ml . CTX has a highest MIC value (8.5) μg/ml compared with the other antibiotics . Virulence-associated genes, cnf-1 ,usp and kpsMTII in the isolated bacteria was detected in the current study , eight isolates appear to be enriched in kpsMTII ( 72.72 % ) of 11 isolates with the high significant differences (p < 0.001 ) , usp genes was identified in 2 isolates (18.18 % ) , while , cnf-1 was undetected in isolated bacteria . Serum cytokines levels for the prostatitis patients and control groups determined by ELISA , high serum concentration of IFN-γ was detected in bacterial prostatitis patients 15.88 ( ± 14.48) pg/ml followed by nonbacterial prostatitis group which records 11.69 (±8.44) pg/ml with high significant differences ( p<0.001 ) than in control group which recorded 5.43 (±1.11) pg/ml . IL-6 elevated in bacterial prostatitis patients 20.11 (±16.76) pg/ml compared with nonbacterial prostatitis patients which records 10.32 (±10.49)pg/ml and control group 3.18 (±1.37) pg/ml with high significant differences (p<0.001). The serum copper concentration was increased in both patients groups , in bacterial prostatitis patients recorded ( 134.51±44.18 ) μg/dl compared with the nonbacterial prostatitis patients which gave ( 127.96±40.24 ) μg/dl . While the control group was recorded ( 84.88±12.86 ) μg/dl with highly significant differences among these groups ( p< 0.001 ) . There is no significant differences shown in the serum zinc concentration among the groups in the current study that recorded ( 130.84±33.09 ) , ( 137.68±37.24 ) and ( 138.31±34.7 ) μg/dl for bacterial prostatitis , nonbacterial prostatitis and control group , respectively .

روابط وملفات

التعريف والنوع

رقم الوثيقة

03e89365-e2bd-41d2-b4cb-b95a4975b43d

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1000294_1.pdf

بيانات النشر

ترجمة العنوان

دراسة جرثومية ومناعية للمرضى المصابين بخمج البروستات في محافظة ذي قار

ألقاب المؤلفين

[{"name_ar":"سعد عبد العزيز عطيه الشبيب","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"عواطف حميد عيسى","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"محمد عبد الكاظم السعدي","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Bacteriological and immunological study of the prostatitis patients in Thi-Qar province

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

prostatitis, IL-6, serum copper, serum zinc, bacterial prostatitis, nonbacterial prostatitis, control group, inflammatory markers, statistical differences

إشراف وإعداد

الإشراف

عواطف حميد عيسى, محمد عبد الكاظم السعدي

الإعداد

سعد عبد العزيز عطيه الشبيب

الاقتباسات الببليوغرافية