The Validity of Latex Agglutination Test as Screening Test for Diagnosis of Toxoplasmosis in HIV Infected Individuals

الموضوعات

طب

الملخص

This study was carried out to assess the validity of the latex agglutination (LAT) as a quickscreening technique against ELISA test in the diagnosis of toxoplasmosis in HIV infectedindividuals. A total number of 72 of HIV/AIDS patients and 72 control sera were collected fromVoluntary Counseling and Testing Center (VCT) and Blood Bank of Sudan Heart Centerrespectively. The HIV infected patients (males and females) were ELISA screened and confirmed byWestern Blot. Anti-Toxoplasma antibodies (IgG and IgM) were detected in all individuals by LATand ELISA. Estimation of CD4+ cells count in patients was carried out.As for the control group serum samples were tested for HIV, VDRL and HBsAg by the blood bankand were negative for HIV, VDRL and HBsAg. The results showed that Anti-Toxoplasma antibodiesdetected by latex agglutination test in HIV positive and HIV negative individuals were detected in43% (31/72) and 38.8%(28/72) respectively. There was no significant difference between the twogroups (x2=0.611, p-value > 0.05). However, the rates of anti-Toxoplasma antibodies detected byELISA in the same groups were; 30.6% (22/72) in HIV positive patients and 18 % (13/72) in HIVnegative individuals. There was significant difference between HIV positive and HIV negativeindividuals(x2=0.001, p-value<0.05). Moreover, the prevalence rate of IgG anti-Toxoplasmaantibodies in HIV positive group was 27.8% (20/72) and in HIV negative group was 8.3%(6/72).Further, the prevalence rates of IgM anti-Toxoplasma antibodies in HIV positive and controlindividuals were; 5.6% (4/72) and 9.7% (7/72) respectively. It was concluded that the prevalencerate of IgG anti-Toxoplasma antibodies was higher in HIV positive than control individuals and thatthe prevalence of anti-Toxoplasma antibodies was higher in HIV infected individuals than in thegeneral population. While the prevalence rate of IgM anti-Toxoplasma antibodies was higher incontrol in HIV positive individuals.iiiThe prevalence rate of anti-Toxoplasma antibodies depending on HIV infection revealed significantdifference. Further, comparison between LAT and ELISA test showed significant difference withELISA being more sensitive than the LAT. Therefore, it was concluded that both tests were useful inthe diagnosis of latent toxoplasmosis and that LAT is a simple and reliable test to be used for quickscreening. However, since it yields some false positive results, the LAT should be confirmed byELISA test which is considered to be a more specific test.

روابط وملفات

التعريف والنوع

رقم الوثيقة

01ec77ad-055b-4c13-94db-1d5614ada0cd

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1640109_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"حامد سليمان","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"سعيدة حسان","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"عثمان عبدالله","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

The Validity of Latex Agglutination Test as Screening Test for Diagnosis of Toxoplasmosis in HIV Infected Individuals

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

حامد سليمان, سعيدة حسان

الإعداد

عثمان عبدالله

الاقتباسات الببليوغرافية