Immunohistochemical and molecular detection of High Risk Human Papilloma Virus (HR-HPV), among Patients with Oral Lesions in Khartoum State

الموضوعات

طب

الملخص

BackgroundHigh Risk human papillomaviruses (HR-HPV), subtypes are strongly linked to etiologyof many human cancers including oral cancer. The epidemiology of infection with different HPVgenotypes greatly varies in different countries. In Sudan, there are considerable numbers of oralcancers, most of them are attributed to the use of Tobacco (toombak) use and alcoholconsumption is the main risk factors for oral cancers (OCs). Other risk factor includes the viralinfection, particularly with Human papilloma virus (HPV).AimThe aim of this study was to identify and genotype the HR-HPV subtypes in oral tissues obtainedfrom Khartoum State with oral lesions.Material and methodsThis is a prospective analytical case control study conducted at Khartoum State during the periodfrom June 2010 to December 2013. Two hundred tissue blocks were retrieved from differenthistopathology laboratories in Khartoum State. Of the 200 formalin fixed paraffin wax processedtissue blocks, 100 were obtained from patients diagnosed with oral cancer and 100 samples wereobtained from patients diagnosed with non-neoplastic lesions. Infection with HPV was initiallydetermined using p16INK4A as a biomarker immunohistochemistry (IHC) method, and thengenotyping was subsequently assessed applying polymerase chain reaction (PCR). Tumor DNAwas amplified using PCR with HR-HPV consensus and multiplex primers. Cytokeratin 19(CK19) antibodies was used to study the expression pattern in normal mucosa, dysplasias,and oral squamous cell carcinomas (OSCC), using IHC.ResultsHPV genomic materials using A6 and A7 primers were detected in 12/200(6%) of oral lesions. Of these, 6/12 (50%) HPV-16, 4/12 (34%) HPV-18, 1/12(8%) HPV-31, and 1/12(8%) HPV-33. Out of the 12 HPV; 8/12(66.7%) HPVwere found in malignant lesions, whereas, 4/12(33.3%) HPV were found inbenign lesions. Consequently, the risk associated with HPV infection wasfound to be statistically significant (P<0.001).The age group 40-49 years was the most susceptible to HPV infection.According to relation between HPV and lesion sites, the most affected sites were buccalmucosa and oropharynx, particularly, SCCs, hence, most of benign lesionswere seen in the salivary glands. The detected HR-HPV were type 16, 18, 31 and 33.The total number of cases detected by the PCR HPV genotyping were 12cases, out of these 11(91.7%), were detected by p16, Statistically, P16 wassignificantly associated with HPV the (P<0.005).ConclusionIn conclusion, the findings of this study provide strong association between HPV and OCs,amongst Sudanese patients. HPV particularly subtypes 16 and 18 play a role in the etiology oforal cancer in the Sudan. On the other hand, in this study P16INK4A immunostaining was usedto screen tissue samples obtained from patients with oral lesions to indicate the presence of HPVinfection. But the exact role of CK19 in the genesis of HPV requires further assessment.

روابط وملفات

التعريف والنوع

رقم الوثيقة

01aa1fac-d434-4fec-965b-591681316fab

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

36239_1.pdf, 36239_2.pdf, 36239_3.pdf

بيانات النشر

ترجمة العنوان

‫الكشف المناعي النسيجي الكيميائي و الجزيئي للسلالات‬ ‫الخطرة لفيروس الورم الحليمي بين المرضى المترددين بآفات‬ ‫الفم في ولاية الخرطوم‬

ألقاب المؤلفين

[{"name_ar":" Ali Yousif Yahia Babiker","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Hussain Gad-Elkarim Ahmed","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Immunohistochemical and molecular detection of High Risk Human Papilloma Virus (HR-HPV), among Patients with Oral Lesions in Khartoum State

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Hussain Gad-Elkarim Ahmed

الإعداد

Ali Yousif Yahia Babiker

الاقتباسات الببليوغرافية