عزل الجراثيم المنتجة للبكتريوسين من حليب أغنام العواس واستخدامها في سلامة الأغذية المتخمرة

الموضوعات

زراعة

روابط وملفات

التعريف والنوع

رقم الوثيقة

0105ac03-9a11-41f2-a0db-cb8beab1cedb

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1965712_1.pdf

بيانات النشر

ترجمة العنوان

Isolating the Bacteriocin-producing Bacteria from "Awassi" Sheep Milk and Using it in the Safety of Fermented Foods

ألقاب المؤلفين

[{"name_ar":"سهيل ابراهيم باشا","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"مني لاوند","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"هادي نور استانبولي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

عزل الجراثيم المنتجة للبكتريوسين من حليب أغنام العواس واستخدامها في سلامة الأغذية المتخمرة

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

The use of Lactic Acid Bacteria(LAB) in food bio-preservation is of great scientific interest in scientific research programs. Recently, a new trend has emerged represented by the use of some bacteriocin-producing LAB or Bacteriocin(s), that possess high specificity of antagonistic activity against Listeria monocytogenes without affecting the remaining natural bacterial load of natural food products.LAB were isolated from (30) raw milk samples, collected from Awassiewes, from the International Center for Agricultural Research in the Dry Areas (ICARDA) (20 samples) and from surrounding areas and villages (10 samples). MRS and M17 Agar media were used, following pour plate methods; where the total number of isolates reached (201) isolate. Their morphology, by using light microscope, revealed that (89%) were Cocci and (11%) were Bacilli.When studying the antagonistic activity of isolates to Listeria monocytogenes (strain 13M/ATCC98A6), by using Spot on-the-lawn method and MRS Agar medium (20g/l D(+)glucose), all isolates showed antagonistic activity against L.monocytogenes through formation of inhibition zones at the place of its colonies, with diameters varying from one isolate to another. The most efficacious antagonistic (isolates that formed inhibition zones of more than 15 mm diameter) were only (29) isolates (15% of the total isolates).The identification of the most efficacious antagonistic isolates, through biochemical and physiological characteristics, and the use of API system, revealed that (27) isolates belonged to Enterococcus faecium (E1, ..., E27), and only (2 isolates belonged to Lactobacillus plantarum((L1, ..., L2).Using modified MRS medium (5g/l D(+)glucose, 100 IU/ml of catalase) to verify bacteriocins production by the most efficacious antagonistic isolates, results revealed significant reduction of the efficacy of most antagonistic isolates to L. monocytogenes, represented by reduction of average diameter of inhibition (≤ 15 mm), as compared with using non-modified MRS Agar medium, except for (3) isolates (E27,E5,E6) which were the most effective (average inhibition diameter >12mm) with (E6) singled out by maintaining its antagonistic efficacy. The efficacy of the (29) isolates antagonistic to Listeriamay be due to their production of bacteriocin(s) according to literatures. The study of isolates’ effect ,that were the most producers of bacteriocin, on L. monocytogenes growth in pasteurized ewes’ milk, revealed an antagonistic effect of isolates on Listeria’s growth which disappeared after (72) hours of incubation. This suggests that theses isolates can be used to ensure the safety of fermented foods, since they are non-pathogenic (non-hemolytic, nor resistant to the antibiotic vancomycin too).

إشراف وإعداد

الإشراف

سهيل ابراهيم باشا, مني لاوند

الإعداد

هادي نور استانبولي

الاقتباسات الببليوغرافية