CRISPR/Cas9-mediated Viral Interference in Plants

الموضوعات

زراعة

الملخص

In prokaryotes, CRISPR/Cas9 system provides molecular immunity to bacteria and archaea against invading phages, conjugative plasmids and nucleic acids. CRISPR/Cas9 system has been adapted for targeted genome editing across diverse eukaryotic species for a variety of applications in basic and applied research. In this dissertation, I propose to adapt the CRISPR/Cas9 system to function as molecular immunity machinery against plant DNA viruses. Therefore, to test whether the CRISPR/Cas9 system is portable to plants, I produced plants stably over-expressing Cas9 and sgRNAs against single or multiple DNA viruses in Nicotiana benthamiana (N. benthamiana) and tomato (Solanum lycopersicum) plants. sgRNAs targeting the Cas9 endonuclease against different coding and non-coding viral sequences were tested in virus- interference experiments. I explored the possibility of generating robust interference against single and multiple DNA viruses. Subsequently, I studied the possibility of virus evasion of the CRISPR/Cas9 machinery and evolution of the virus escapees. Finally, I produced N. benthamaiana and tomato plants stably expressing the CRISPR/Cas9 machinery for developing durable virus resistance. Furthermore, developing effective viral-interference system in plants will help to understand the molecular underpinning of virus biology and host-defense mechanisms against plant viruses.In conclusion, my research project attempted to establish the efficacy and extend the utility of CRISPR/Cas9 system for viral interference in plants which promise exciting applications including producing engineered plants resistant to multiple viral infection.

روابط وملفات

التعريف والنوع

رقم الوثيقة

008aea7d-2506-47ac-84bb-162c408a31fc

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1699102_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"مجدي محمد محفوظ ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"منال طشقندي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

CRISPR/Cas9-mediated Viral Interference in Plants

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

CRISPR/Cas9 , crop engineering , resistance to virus , viral interference

إشراف وإعداد

الإشراف

مجدي محمد محفوظ

الإعداد

منال طشقندي

الاقتباسات الببليوغرافية