Peste Des Petits Ruminants (Ppr) In Sudan Detection, Virus Isolation And Identification P A T H O G E N I C I T Y A N D S E R O S U R V E I L L A N C E

الموضوعات

طب

الملخص

The reference PPRV, the Sudanese isolate SUD 72/1 (Sinnar) BKC. P.4, LTC. P.2was found to have a titre of 105.4 TCID50 /ml. Using the reference PPRV, hyperimmuneserum was prepared in goats, and detected for PPRV antibodies by AGPT and C-ELISA.Forty lymph nodes and spleen samples from suspected cases of PPR in sheep andgoats were tested by AGPT and HA tests for detection of PPRV antigen. The results of bothtests revealed that HA test was more sensitive than AGPT for detection of PPRV antigen.The HA test was carried out using chicken, goat and pig RBCs. Chicken RBCs wasfound to be the most sensitive for detection of PPRV antigen, followed by goat then pigRBCs.PPRV was isolated from 13 infected sheep and goats out of 40 suspected cases ofsheep and goats in LKC and LTC. Three PPRV isolates were from Khartoum State; eightisolates were from CVRL, Soba, Khartoum State; one isolate was from Gezira State andone isolate was from Bashaier, River Nile State. The isolates were identified by AGPT andHA tests. Typical PPR CPE was produced by the isolated viruses and a single precipitationline was demonstrated in the AGPT after treatment of the infected culture fluid with 2%SDC. The CPE of the 1st passage of PPRV appeared within 8-12 days p.i. while in the 2nd-4th passages the CPE appeared within 4-7 days and took 10-25 days to involve most of thecell sheet.Sixteen goats were experimentally infected with PPRV isolates. The 4th passage oftwo PPRV isolates (Bashaier and Soba isolates) in tissue culture cells were used forexperimental inoculation of group (A) and (B), each goat received 6×10 4.5 TCID 50 /ml6×10 4.3 TCID 50 /ml of Bashaier and Soba isolates, respectively. The 20% infected tissuesuspensions of the goat propagated isolates (Khartoum and Soba isolates) were used forinoculation of group (C) and (D), each goat received 6 ml×20% suspension of the virus.The inoculated goats showed typical PPR clinical signs, gross lesions and histopathologicalchanges while control animals (group E) appeared healthy. Two goats from group (A) diedon the 16th and 19th days post inoculation, respectively. PPRV was reisolated in LKC andLTC and detected by HA test. It was detected in lacrymal fluid and nasal swabs collectedon the 6th and 7th day p.i.Serum samples from 16 goats experimentally infected with PPRV isolates on the 7,10, 14, 18, 21, 28 and 30 days p.i were tested for PPRV antibodies by C-ELISA. Traces ofPPRV antibodies were shown on day 7 and they continued to rise till the 28th day anddropped on the 30th day which is as the 9th day post challenge.Serum samples were collected from sheep and goats from six different States in theSudan namely Khartoum, River Nile, Blue Nile, Darfur, Kordofan and Southern States andtested for PPRV antibodies by CIEP and C-ELISA. The result of both tests showed highprevalence of PPRV antibodies in the six States. Using CIEP, the highest prevalence ofPPRV antibodies was in River Nile State and the lowest prevalence was in Kordofan State.When using C-ELISA, the highest prevalence of PPRV antibodies was in Blue Nile Stateand the lowest prevalence was in River Nile State. The CIEP technique was shown to bemore sensitive than the C-ELISA technique but there was inconsistency in the resultsobtained from both techniques.

روابط وملفات

التعريف والنوع

رقم الوثيقة

002e0d67-00a7-45a6-8a46-4f5a8101b132

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1639366_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"عبدالواحد سعيد","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"محاسن النور","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"نصيبة احمد","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

French

المصدر والدورية

اسم المصدر

Peste Des Petits Ruminants (Ppr) In Sudan Detection, Virus Isolation And Identification P A T H O G E N I C I T Y A N D S E R O S U R V E I L L A N C E

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

عبدالواحد سعيد, محاسن النور

الإعداد

نصيبة احمد

الاقتباسات الببليوغرافية