Detection of mutations in drugs resistance genes Mycobacterium tuberculosis in Khartoum state

الموضوعات

طب

الملخص

In this study, the molecular biological technique was used to studythe drugs resistance in 120 samples of Mycobacterium tuberculosis(M.tb) isolated from Khartoum State.A systematic random samples were isolated from patients withsputum smear positive for acid-fast bacilli (AFB), from relapsed,treatment failed, defaulted, and new cases under treatment for morethan two months. Sputum samples were initially subjected to standardmicroscopic and cultural techniques for tentative identification, thenconfirmed using dna J gene analysis.Ninety-five out of the 120 samples revealed positive growth on L Jmedia incubated at 37°C for up to 6 weeks. By using dnaJ geneanalysis, 50 (52.6 % ) out of 95 were found to be Mycobacteriumtuberculosisand four (4.2 %) belong to mycobacteria other thantuberculosis (MOTT ).The Mycobacterium tuberculosis( 50 samples)were subjected to PCR-based dot blothybridization technique by usingradio-labeled P32to detect mutations in different loci in six genes (katG315, rpoB 531, rpsL 43, rrs 513 & rrs 491 and embB 306 ) associatedwith drug resistance to isoniazid, rifampicin, streptomycin andethambutol respectively. Mutations genes were identified in 12% ( katG5gene ), 8% (rpoB gene ), 20% (rpsL),10 % (rrs genes ) and 6 %.(embBgene ) associated with isoniazid, rifampicin, streptomycin andethambutol respectively, and 4% were multi-drug resistance tuberculosis( MDR-TB). Among the fifty tuberculosis patients, Thirty nine (78%) ofthem had problematic tuberculosis distributed as follow: The treatmentfailed cases (36 %) had mutations associated with four genes, katG (5.6%), rrs (11.1 %), rpsL (16.7 %), and rpoB (5%) The relapsed cases(28%) had mutations associated with tow genes, katG gene (14.3 %)and rrs gene (21.4 %).The defaulted cases (14 %) had mutations associated with fourgenes, katG (28.6 %), rrs ( 42.9 % ), rpsL (14.3 % ), and embB (42.9% ).Twenty two percent of newly diagnosed patients were found to beresistant to rifampicin and also resistance to streptomycin associatedwith three mutant genes, rpoB (27.3) and rrs (27.3 %) and rpsL ( 9.10 %)respectively.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0018aa3e-c20a-4008-a02d-dcd19eb3a08e

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf image

أسماء الملفات

0018aa3e-c20a-4008-a02d-dcd19eb3a08e_1.pdf

بيانات النشر

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Detection of mutations in drugs resistance genes Mycobacterium tuberculosis in Khartoum state

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

نجيب سعيد

الإعداد

جهاد سليمان شرف الدين

الاقتباسات الببليوغرافية