تأثير مصدر الخزعة النباتية في التباين الوراثي للنبيتات الناتجة عن زراعة المعلقات الخلوية في صنفين من البطاطا

الموضوعات

زراعة

روابط وملفات

التعريف والنوع

رقم الوثيقة

000752de-98bb-4e84-b699-e6d76bc098cc

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1963694_1.pdf

بيانات النشر

ترجمة العنوان

BiotechnologyEffect of explants on genetic variability of the plantlets produced from cell suspension in two potato cultivars

ألقاب المؤلفين

[{"name_ar":"خالد عبد الرزاق المحمد","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"خليل المعري","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"فهد البيسكي","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

تأثير مصدر الخزعة النباتية في التباين الوراثي للنبيتات الناتجة عن زراعة المعلقات الخلوية في صنفين من البطاطا

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

Thisresearch was carried out in the laboratories ofTheNational Commission for Biotechnology (NCBT) and The Faculty of Agriculture, Damascus University(Damascus-Syria)during the years2015-2016. It aimed to culture and regenerate cellular suspensions of two 4Npotato cultivars Solanum tuberosumL. var Amarin and Solanum tuberosum L. var Challenger, whichwere locally certifiedin 2013. Genetic variations were evaluated on organic callus formed from cellular suspensions using ISSR.Callus of both cultivars (Amarin and Challenger) wasinitiated starting with two sources of explants (leaves and internodes) using Murashig and Skooge (MS) medium supplemented with different concentrations oftheauxin 2,4-dechlorophenoxy acetic acid (2,4-D).The best concentrations of2,4-Dwas 3 mg/ l, for callus initiation of cultivar Amarin 96%and 92% from Leaves and Internodes respectively, and 92% of cultivar Challenger from both explants.Cell suspensions were cultured from callus into liquid medium ½ MS containing 3 mg/ l 2,4-D. Some growth parameters (cellular density, mitotic index, andcellviability) were studied. Cell suspensions of both cultivars reached stability phase in the third week of culturing.Cellular density of cell suspensions has increased significantly inthecultivar Amarin in the third and fourth weeks, while it increased gradually in the cultivar Challenger. Division ratios of internodes-cellular suspensions in the cultivar Amarin were higher comparing with those of leaves, while no-significant differences were observed in the cultivar Challenger. Cellviability ofthecell suspensions of the cultivar Amarin was higher in the third and fourth weeks comparing with the Challenger cultivar. Different concentrations of growth regulators (6-Benzyl amino purin (BAP) and Zeatin (Z))were used in order to regenerate cell suspensions, and the highest 118percentage of the formed callus from cellular suspensions of both cultivars was obtained using 1.75 mg / l ofBAP. It was observed that Z had less effectiveness than BAP in the regeneration of callus from the cellular suspensions; cellular suspensions varied in their responds to the regeneration in the presence of Z, that the optimal callus regeneration was obtained using 1.5 mg/l Z starting from leaves and internodes cellular suspensions in Amarin cultivar and from leaves cellular suspensions in Challenger cultivar. The effect of the explant source (leaves or internodes) on the genetic variations among mother plants and organic callus formed from cellular suspensions was the same in Amarin cultivar using BAP or Z in the regeneration medium; while in the cultivar Challenger genetic variations among mother plants and organic callus was higher starting with leaves comparing with internodes using Z in the regeneration medium.Genetic variation in both studied cultivars (Amarin and Challenger) and the using BAP in the regeneration medium was higher comparing with it using Z

كلمات الباحثين

callus, cellular suspensions, genetic variation, potato, بطاطا, تباين وراثي, معلقات الخلوية

إشراف وإعداد

الإشراف

خليل المعري, فهد البيسكي

الإعداد

خالد عبد الرزاق المحمد

الاقتباسات الببليوغرافية